Each 4.5 mL venous blood sample in a 5.0 mL BD blood collection tube with 0.5 mL 3.2% sodium citrate (Catalog No. 366415, Becton Dickinson, Franklin Lakes, NJ, USA) was centrifuged at 1,500 g for 10 minutes (KUBOTA 4000 centrifuge, Fujioka Japan). Using the SYSMEX CA-1500 Coagulation Analyzer (Kobe, Japan), the plasma obtained was quantified for six coagulation parameters including prothrombin time (PT) (Dade INNOVIN, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany), activated partial thromboplastin time (APTT) (Dade Actin FSL, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany), and fibrinogen (Dade Fibrinogen Determination Reagents, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany) using the clotting method as well as D-dimer with the immuno-turbidity approach (INNOVANCE D-dimer, Siemens Healthcare Diagnostics Products, GmbH. 35041 Marburg/Germany), antithrombin III (Siemens Antithrombin III assay, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany) and protein C (Dade Behring Protein C Reagent, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany) using the chromogenic method according to the manufacturer's instructions.
Dade fibrinogen determination reagents
Manufactured by Siemens
Sourced in Germany
The Dade Fibrinogen Determination Reagents are a set of laboratory test kits used to measure the concentration of fibrinogen in human blood samples. The reagents provide the necessary components to perform the quantitative determination of fibrinogen levels. The core function of these reagents is to enable the assessment of fibrinogen, a protein involved in blood clotting.
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Lab products found in correlation
Innovance d dimer, by Siemens (1 mentions)
Dade actin fsl, by Siemens (1 mentions)
Dade innovin, by Siemens (1 mentions)
Chloral hydrate, by Tianjin Damao (1 mentions)
Calcium chloride solution, by Siemens (1 mentions)
Test thrombin reagent, by Siemens (1 mentions)
Dade actin activated cephaloplastin reagent, by Siemens (1 mentions)
2 protocols using dade fibrinogen determination reagents
1
Coagulation Profile Assessment of Venous Blood Samples
2
Characterization of Moutan Cortex Charcoal Compounds
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Reagents All the chemicals used in the experiments of this study were of reagent grade and obtained from commercial suppliers: Dade Actin Activated Cephaloplastin Reagent, Calcium Chloride Solution, Dade Fibrinogen Determination Reagents, Test Thrombin Reagent and Detergent for Fully Automated Blood Coagulation Analyzer (all from Siemens Healthcare Diagnostics Products GmbH, Germany); Disposable Vacuum Blood Vessels (SANLI, China); Chloral Hydrate (Damao Chemical Reagent Factory, Tianjin, China).
New Zealand white rabbits purchased from Laboratory Animal Center, Guangzhou University of Chinese Medicine (License No: SCXK (YUE) 2016-0041, Guangzhou, China); Compounds (M3, M9, M10, M11, M12, M15, M24, M31) were extracted, isolated and purified from Moutan Cortex Charcoal in our laboratory. The purity of the isolated components was shown to be higher than 96% by HPLC, and their structures were elucidated by comparing their spectroscopic data (electrospray ionization (ESI)-MS, 1 H-NMR and 13 (link) C-NMR, heteronuclear multiple bond connectivity (HMBC), 1 H-detected heteronuclear multiple quantum coherence (HMQC) and H-H correlation spectroscopy (COSY)) in literature. 29, 30) Structures of the eight compounds are shown in Fig. 2.
New Zealand white rabbits purchased from Laboratory Animal Center, Guangzhou University of Chinese Medicine (License No: SCXK (YUE) 2016-0041, Guangzhou, China); Compounds (M3, M9, M10, M11, M12, M15, M24, M31) were extracted, isolated and purified from Moutan Cortex Charcoal in our laboratory. The purity of the isolated components was shown to be higher than 96% by HPLC, and their structures were elucidated by comparing their spectroscopic data (electrospray ionization (ESI)-MS, 1 H-NMR and 13 (link) C-NMR, heteronuclear multiple bond connectivity (HMBC), 1 H-detected heteronuclear multiple quantum coherence (HMQC) and H-H correlation spectroscopy (COSY)) in literature. 29, 30) Structures of the eight compounds are shown in Fig. 2.
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