Anti pp2a cα β

HeLa cells were obtained from RIKEN Cell Bank and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum. HeLa cells stably expressing the FLAG-HA-nuclear localization signal (NLS)-fused canine BRCA2 BRC repeats were generated.
Western blotting was performed using the following antibodies: anti-RAD51 (dilution 1:10,000, Bio Academia, Osaka, Japan), anti-PP2A-Cα/β (dilution 1:1000, Santa Cruz, CA, USA), anti-FLAG (2H8; dilution 1:1000, Trans Genic, Kobe, Japan), anti-FLAG (PM020; 1:1000, MBL, Nagoya, Japan), and anti-Lamin B1 (dilution 1:2000 MBL).

Rat cortical neurons were washed once with ice-cold PBS and homogenized in cold NP-40 buffer (1% Nonidet P-40, 50 μM Tris–HCl, 5 mM EDTA, and protease inhibitor cocktail). The primary antibodies were anti-PP2A-Cα/β (1:1,000; Santa Cruz Biotechnology), anti-α-tubulin (1:1,000; Sigma-Aldrich). Western blots are done for three independent experiments.