After oxygen consumption measurement, individual islets in the XF96 spheroid plate were imaged in brightfield (10× objective) with the Operetta high-throughput imaging system (Perkin Elmer, Waltham, MA). Islet images were analyzed in ImageJ by manually drawing a line around the perimeter of each islet with the “Polygon selection” drawing feature and selecting the “Measure” function under the “Analyze” tab.
Operetta high throughput imaging system
Manufactured by PerkinElmer
The Operetta high-throughput imaging system is a laboratory instrument designed for automated cell-based assays and high-content screening. It features a modular design and offers multiple imaging modes, including fluorescence and label-free imaging. The system is capable of capturing high-resolution images of cells and tissues in microplates, enabling researchers to analyze cellular morphology and biological processes in a high-throughput manner.
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Lab products found in correlation
3 protocols using operetta high throughput imaging system
1
Islet Imaging and Morphometric Analysis
2
Quantifying CRISPR Efficiency in HEK293T Cells
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HEK293T cells were transfected with pCAG-EGxxFP-β-actin and pTagRFP-C together with pCGSapI-β-actin-sgRNA#1 or - sgRNA#2. Forty-eight hours after transfection, cells were fixed with 4% paraformaldehyde (PFA) and stained with Hoechst 33342 (WAKO). Fluorescence images were taken with the Operetta high throughput imaging system (Perkin Elmer). To quantify ratio of EGFP positive cells to TagRFP positive cells, the images were analyzed by Operetta imaging software 2.6 (Perkin Elmer).
3
Cell Nuclei Labeling and Imaging
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Following treatment, cell nuclei were labeled with Hoechst 33324 (10 µM, 10 min, Sigma-Aldrich). The Operetta highthroughput imaging system (Perkin Elmer) was used for cell counting. Five fields were imaged per well. Image analysis was performed by using the Columbus Image Analysis Software (Perkin Elmer).
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