Pgk egfp ppm1h wt

Plasmids used include PGK EGFP-LC3 (Addgene plasmid #200427; subcloned from PGK mCherry-LC3B, gift from Michael Ward, National Institutes of Health, Maryland), PGK 4xMito-mEmerald (Addgene plasmid #200430; subcloned from 4xMito-mScarlet-I, Addgene plasmid #98818), PGK ARF6^Q67L-CFP (Addgene plasmid #200428; subcloned from CMVARF6^Q67L-CFP, Addgene plasmid #11387), PGK CFP (Addgene plasmid #200429; subcloned from CMV ARF6^Q67L-CFP, Addgene plasmid #11387), CMV mScarlet-LC3B (Addgene plasmid #200431; subcloned from EGFP-LC3B, gift from T. Yoshimori, Osaka University, Japan, with mScarlet from Addgene plasmid #85054), PGK mScarlet-LC3B (Addgene plasmid #200083; subcloned from CMV mScarlet-LC3B, Addgene plasmid #200431), CMV EGFP-PPM1H-WT (#DU62939, MRC PPU reagents and services, University of Dundee, Scotland), PGK EGFP-PPM1H-WT (Addgene plasmid #200084, subcloned from #DU62939, MRC PPU reagents and services, University of Dundee, Scotland), CMV EGFP-PPM1H-H153D (subcloned from pLVX HA PPM1H H153D #DU66215, acquired from MRC PPU reagents and services, University of Dundee, Scotland), CMV FLAG-LRRK2-R1441G (#DU13077, MRC PPU reagents and services, University of Dundee, Scotland), PB-TO-hNGN2 (gift from iPSC Neurodegenerative Disease Initiative (iNDI) & Michael Ward, Addgene plasmid #172115), and piggyBac^™ transposase vector (available from Transposagen).