Human GC cell lines, including MGC803, MKN1, HGC‐27, AGS, NUGC4, and AZ521 were purchased from the American Type Culture Collection (ATCC). All cell lines were cultured at 37 °C with 5% CO2 in RPMI‐1640 medium (GIBCO) supplemented with 10% fetal bovine serum (FBS, GIBCO, Thermo Scientific), penicillin (100 IU/ml), and streptomycin (100 mg/mL). The USP7 inhibitor FT671 and P5091 were purchased from TargetMol (FT671, cat#1959551‐26‐8; P5091, cat# 882257‐11‐6). The investigated compounds, including DHPO, were obtained from a natural product library established in Prof. Wei‐Dong Zhang's laboratory, with purity being >95%.[19 (link),
20 (link)
]A total of 128 pairs of fresh‐frozen GC tumor tissues and para‐tumor samples were collected from patients who underwent GC surgery in the Department of Gastric Surgery at Zhejiang Cancer Hospital (Hangzhou, China). Informed consent forms were signed in advance. Follow‐up was conducted in time to record the survival time of patients. All studies associated with clinical samples and data were carried out following the principles of the Declaration of Helsinki and were approved by the Ethics Committee of Zhejiang Cancer Hospital (study number: IRB‐2021‐456).
20 (link)
]A total of 128 pairs of fresh‐frozen GC tumor tissues and para‐tumor samples were collected from patients who underwent GC surgery in the Department of Gastric Surgery at Zhejiang Cancer Hospital (Hangzhou, China). Informed consent forms were signed in advance. Follow‐up was conducted in time to record the survival time of patients. All studies associated with clinical samples and data were carried out following the principles of the Declaration of Helsinki and were approved by the Ethics Committee of Zhejiang Cancer Hospital (study number: IRB‐2021‐456).