Fmoc rink amide am resin

Protected amino acids, coupling agents (HATU, Oxyma), and Fmoc-Rink Amide AM resin used for peptide synthesis were purchased from IRIS Biotech GmbH (Marktrewitz, DE). Solvents, including acetonitrile (CH3CN) and dimethylformamide (DMF) were purchased from Carlo Erba reagents (Milan, Italy). Other products such as trifluoroacetic acid (TFA), sym-collidine, diisopropylethylamine (DIPEA), piperidine, were from Sigma-Aldrich (Milan, Italy). HPLC analyses for peptides characterization were performed on an Alliance HT WATERS 2795 system, equipped with a PDA WATERS detector 2996, whereas preparative purifications were carried out on a WATERS 2545 preparative system (Waters, Milan, Italy) fitted out with a WATERS 2489 UV/Visible detector.

Protected amino acids, Fmoc Rink Amide AM resin, and coupling reagents were purchased from Iris Biotech. All other chemicals were Sigma-Aldrich products and were used without further purification. DMF was always previously fluxed with nitrogen when used in the synthesis of peptides. Cotton samples were supplied by Santex Spa (medical gauze cotton, G-cotton) and Piave Maitex Srl (organic cotton for textiles, T-cotton). The T-cotton did not require further treatments prior to use, while the G-cotton needed to be mercerized prior to use.

All Fmoc-protected amino acids, N,N-diisopropylcarbodiimide (DIC), OxymaPure^®, and Fmoc-Rink Amide AM resin (0.6 mmol/g) were purchased from Iris Biotech GmbH (Marktredwitz, Germany). Solvents for synthesis, deprotection reagents, and cleavage reagents used were of synthesis grade and purchased from Merck KGaA (Darmstadt, Germany). Solvents and other chemicals used for high-performance liquid chromatography (HPLC), electrospray ionization (ESI) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and circular dichroism (CD) spectroscopy analyses were of HPLC reagent grade and purchased from Merck KGaA (Darmstadt, Germany).