FP mass was determined by liquid chromatography-mass spectrometry (LC-MS), where the protein column eluant was directly injected into the electrospray interface of an Orbitrap XL (Thermo Fisher). FP precipitation was minimized by using a steep gradient and injecting directly into the mass spectrometer. The 1100 LC (Agilent Technologies) had solvent A = 2% acetonitrile (ACN), 0.1% formic acid (FA) and solvent B = 90% ACN, 0.1% FA, the flow rate was 80 µl/min, and the gradient was 18% A to 100% B in 2.4 minutes. Protein was eluted within 10 min from a 1 mm inner diameter, 50 mm long PLRP-S C18 column (Agilent Technologies). Orbitrap IonSpray (electrospray) interface had a sheath gas flow rate of 34 and T = 275 °C. Capillary was 39 V and the tube lens was 140 V. Full scan, mass spectra were collected in the ion trap and Fourier transform profile mode, with Orbitrap resolution 60,000, from 500 to 1,800 mass-to-charge units. Protein mass spectra were deconvolved using either the extract_msn program (Xcalibur software) or with ProMassCalc (ThermoFisher).
Promasscalc
Manufactured by Thermo Fisher Scientific
ProMassCalc is a software tool developed by Thermo Fisher Scientific that enables users to calculate the theoretical mass of biomolecules, such as proteins, peptides, and oligonucleotides, based on their primary sequence. The software utilizes established algorithms to provide accurate mass calculations, allowing researchers to verify experimental mass spectrometry data and support their biomolecular analysis workflows.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using promasscalc
1
Protein Mass Spectrometry Profiling
2
Protein Mass Spectrometry Profiling
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FP mass was determined by liquid chromatography-mass spectrometry (LC-MS), where the protein column eluant was directly injected into the electrospray interface of an Orbitrap XL (Thermo Fisher). FP precipitation was minimized by using a steep gradient and injecting directly into the mass spectrometer. The 1100 LC (Agilent Technologies) had solvent A = 2% acetonitrile (ACN), 0.1% formic acid (FA) and solvent B = 90% ACN, 0.1% FA, the flow rate was 80 µl/min, and the gradient was 18% A to 100% B in 2.4 minutes. Protein was eluted within 10 min from a 1 mm inner diameter, 50 mm long PLRP-S C18 column (Agilent Technologies). Orbitrap IonSpray (electrospray) interface had a sheath gas flow rate of 34 and T = 275 °C. Capillary was 39 V and the tube lens was 140 V. Full scan, mass spectra were collected in the ion trap and Fourier transform profile mode, with Orbitrap resolution 60,000, from 500 to 1,800 mass-to-charge units. Protein mass spectra were deconvolved using either the extract_msn program (Xcalibur software) or with ProMassCalc (ThermoFisher).
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