Seventy-two hours post-transfection, total protein was extracted from SH-SY5Y and SK-N-SH cells. Western blotting was performed to detect the expression of target proteins. The primary antibodies, including anti-extracellular signal-regulated protein kinase 1/2 (ERK1/2), anti-phospho (p)-ERK1/2 (Thr202/Tyr204), anti-c-Jun N-terminal kinase (JNK), anti-p-JNK (Thr183/Tyr185), anti-p38, anti-p-p38 (Thr180/Tyr182), anti-phosphatidylinositol 3-kinase (PI3K), anti-p-PI3K (Tyr458/Tyr199), anti-Akt, anti-p-Akt (Ser473), anti-mammalian target of rapamycin (mTOR), and anti-p-mTOR (Ser2448) antibodies were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Anti-PCNP, Anti-B-cell lymphoma-2 (Bcl-2), anti-Bcl-2-associated X protein (Bax), anti-B-cell lymphoma-extra large (Bcl-xl), anti-Bcl-xl/Bcl-2-associated death promoter (Bad), anti-cleaved caspase-3, anti-cleaved caspase-8, anti-cleaved caspase-9, anti-Cleaved poly adenosine diphosphate-ribose polymerase (PARP), and anti-GAPDH antibodies were purchased from ProteinTech (Chicago, IL, USA). The horseradish peroxidase-conjugated secondary antibody was purchased from Cell Signaling Technology. The results were normalized to the level of GAPDH. The reaction was visualized using an enhanced chemiluminescence system (Thermo Fisher Scientific, Rockford, IL, USA). The bands were semi-quantified with Image J software.
Anti cleaved caspase 8
Manufactured by Proteintech
Sourced in United States
Anti-cleaved caspase-8 is a laboratory reagent used for the detection of the cleaved form of caspase-8 protein. Caspase-8 is a protease enzyme involved in the initiation of apoptosis, or programmed cell death. The anti-cleaved caspase-8 antibody can be used to identify and quantify the activated, cleaved form of this enzyme in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence system, by Thermo Fisher Scientific (2 mentions)
Anti cleaved poly adenosine diphosphate ribose polymerase parp, by Proteintech (2 mentions)
Anti cleaved caspase 9, by Proteintech (2 mentions)
Anti cleaved caspase 3, by Proteintech (2 mentions)
Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Anti pcnp, by Proteintech (1 mentions)
Anti p mtor ser2448, by Cell Signaling Technology (1 mentions)
Anti mammalian target of rapamycin, by Cell Signaling Technology (1 mentions)
Anti p akt ser473, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Anti caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by Affinity Biosciences (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Anti parp1, by Proteintech (1 mentions)
Anti chop, by Proteintech (1 mentions)
Anti caspase 8, by Proteintech (1 mentions)
Anti bcl xl, by Proteintech (1 mentions)
Anti cytochrome c, by Proteintech (1 mentions)
Anti grp78, by Proteintech (1 mentions)
Anti h ras, by Cell Signaling Technology (1 mentions)
3 protocols using anti cleaved caspase 8
1
Western Blot Analysis of Signaling Proteins
2
Antibody-based Protein Expression Analysis
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Anti-HA antibody was from Huaxingbio (Beijing, China); anti-caspase3 antibody was from Cell Signaling Technology (Danvers, MA, USA); anti-caspase 8, anti-caspase 4, anti-cleaved-caspase 3, anti-Bcl-XL, anti-cleaved caspase 8, anti-cytochrome C, anti-CHOP, anti-PARP1, and anti-GRP78 antibodies were all purchased from Proteintech Group, Inc. (Rosemont, IL, USA); anti-caspase 10 antibody was from Bioworld Technology, Inc. (St. Louis Park, MN, USA); anti-β-actin antibody was from Transgen Biotechnology (Beijing, China); anti-GAPDH antibody was from Affinity Biosciences (Cincinnati, OH, USA). Human recombinant IFNalpha-1a was purchased from ProSpec-Tany TechnoGene (Ness Ziona, Israel). Cell counting kit-8 was from Dojindo (Kumamoto, Japan).
3
Western Blot Analysis of MAPK Pathway
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Total protein was extracted from SMMC-7721 and Huh-7 cells. Western blotting was performed to detect the expression levels of target proteins. The primary antibodies, including anti-H-RAS, anti-RAF, anti-phospho (p)-c-Raf (Ser259), anti-MEK1/2, anti-p-MEK1/2 (Ser217/221), anti-extracellular signal-regulated protein kinase 1/2 (ERK1/2), and anti-p-ERK1/2 (Thr202/Tyr204) antibodies were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Anti-B-cell lymphoma-2 (Bcl-2), anti-Bcl-2-associated X protein (Bax), anti-B-cell lymphoma-extra large (Bcl-xl), anti-Bcl-xl/Bcl-2-associated death promoter (Bad), anti-cleaved caspase-3, anti-cleaved caspase-8, anti-cleaved caspase-9, anti-cleaved poly adenosine diphosphate-ribose polymerase (PARP), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from ProteinTech (Chicago, IL, USA). The horseradish peroxidase-conjugated secondary antibody was purchased from CST. The results were normalized to the level of GAPDH. The reaction was visualized using an enhanced chemiluminescence system (Thermo Fisher Scientific, Rockford, IL, USA). The bands were semi-quantified with Image J software.
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