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3 protocols using nh4oh 25

1

Heavy Metal Removal Using Chitosan

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CuSO4·5H2O, CdSO4, ZnSO4, Pb(NO3)2, Cu(NO3)2 ·3 H2O, Cd(NO3)2 ·4H2O, Zn(NO3)2, NaCl, chitosan, and glacial acetic acid (analytical grade, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) were used in the studies. NaOH pellets, H2SO4 (96%), HCl 35% ultrapure and NH4OH 25% (analytical grade) were purchased from Merck KGaA Darmstadt, Germany.
Ultrapure water was used for preparing the feed solutions.
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2

Synthesis of Magnetic Adsorbents for Dye Removal

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Fe(NO3)3·9H2O 96%, Co(NO3)2·6H2O, chitosan, and glacial acetic acid (analytical grade, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) were used in the synthesis of magnetic materials. NaOH pellets, HCl 35% suprapure and NH4OH 25% (analytical grade) were purchased from Merck KGaA Darmstadt, Germany. Congo Red (CR) 99% (C32H22N6Na2O6S2) and Methyl Orange (MO) (ACS reagent, dye content 85 %, C14H14N3NaO3S, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) and ultrapure water were used for preparing the dye solutions. Ethyl alcohol (95%) and acetone (99%) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) were used as desorbing agents, while ammonium acetate 99% and acetonitrile 99% (Merck KGaA Darmstadt, Germany) as mobile phase for chromatographic separation and detection were used.
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3

Cobalt Ferrite Preparation and Dye Adsorption

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The cobalt ferrite samples were prepared by the use of Fe(NO3)3·9H2O 99%, Co(NO3)2·6H2O and sodium bis-2-ethyl-hexyl sulfosuccinate (analytical grade, Sigma Aldrich, St. Louis, MO, USA). NH4OH 25% and HCl 35% suprapure (analytical grade, Merck KGaA Germany, Darmstadt, Germany) were used to adjust the solution’s pH. The dye solutions of desired concentrations were obtained from Congo Red (CR) 99% (C32H22N6Na2O6S2) and Methyl Orange (MO) (ACS reagent, Dye content 85%, C14H14N3NaO3S, Sigma Aldrich). Ethyl alcohol (95%) (Sigma Aldrich, St. Louis, MO, USA) was applied as a desorbing agent. Ammonium acetate 99% and acetonitrile 99% (Merck KGaA Germany, Darmstadt, Germany) were utilized as a mobile phase for the dye’s chromatographic separation and detection.
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