The UV-Vis absorbance of DHA-dFdC, DHA, dFdC, and the physical mixture of dFdC and DHA, all dissolved in methanol, was evaluated using a BioTek Synery HT Multi-Mode Microplate Reader (Winooski, VT) using the scanning mode. Modulated differential scanning calorimetry (DSC) was used to evaluate the thermal properties of DHA-dFdC. Samples (2-4 mg) were placed in sealed pans, and the DSC analysis was carried out using DSC Q200 (TA Instruments, New Castle, DE) at a ramp rate of 5°C/min under nitrogen flow. X-ray diffraction (XRD) analyses of DHA-dFdC, dFdC, DHA, and the physical mixture of dFdC and DHA (1:1, m/m) was carried out in the x-ray facility in the Department of Chemistry at the University of Texas at Austin using a Rigaku Spider single-crystal x-ray diffractometer (Rigaku, Tokyo, Japan).
Synery ht multi mode microplate reader
Manufactured by Agilent Technologies
Sourced in United States
The Synergy HT Multi-Mode Microplate Reader is a compact and versatile instrument designed for a wide range of microplate-based assays. It features multiple detection modes, including absorbance, fluorescence, and luminescence, enabling researchers to perform various analytical techniques within a single platform.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using synery ht multi mode microplate reader
1
Analytical Characterization of DHA-dFdC Conjugate
2
Nanoparticle-Mediated Drug Release Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The rate at which DOX
was released from nanoparticles was measured
as a function of time when the nanoparticles were incubated in phosphate-buffered
saline (PBS, pH 7.4 or 6.8, 10 mM). Triplicate samples of 5 mg of
nanoparticles were suspended in 0.5 mL of PBS and sonicated briefly
in an ultrasonic water bath. The samples were then incubated in an
orbital shaker at 37 °C, 100 rpm. At various time points, the
particles were centrifuged at 13 000 × g for 5 min, and 100 μL of supernatant was removed and replaced
with fresh PBS. The fluorescence intensity of DOX in the supernatant
was measured using a BioTek Synery HT Multi-Mode Microplate Reader
(Winooski, VT, USA) at excitation 470 nm/emission 590 nm to determine
the DOX released from nanoparticles.
was released from nanoparticles was measured
as a function of time when the nanoparticles were incubated in phosphate-buffered
saline (PBS, pH 7.4 or 6.8, 10 mM). Triplicate samples of 5 mg of
nanoparticles were suspended in 0.5 mL of PBS and sonicated briefly
in an ultrasonic water bath. The samples were then incubated in an
orbital shaker at 37 °C, 100 rpm. At various time points, the
particles were centrifuged at 13 000 × g for 5 min, and 100 μL of supernatant was removed and replaced
with fresh PBS. The fluorescence intensity of DOX in the supernatant
was measured using a BioTek Synery HT Multi-Mode Microplate Reader
(Winooski, VT, USA) at excitation 470 nm/emission 590 nm to determine
the DOX released from nanoparticles.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!