P xylene bis pyridinium bromide dpx

EYPC was kindly donated by NOF Co. (Tokyo, Japan). 1,2-Cyclohexanedicarboxylic anhydride, OVA, fluorescein isothiocyanate (FITC), p-xylene-bis-pyridinium bromide (DPX) were purchased from Sigma (St. Louis, MO, USA). Dextran having molecular weight of 70,000, 1-aminodecane, pyranine and Triton X-100 were obtained from Tokyo Chemical Industries Ltd. (Tokyo, Japan). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl morpholinium chloride (DMT-MM) was from Wako Pure Chemical Industries Ltd. (Osaka, Japan). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) was from Life Technologies. Dialysis tube (molecular weight cutoff: 10 kDa) was purchased from Sekisui Medical Co., Ltd. (Tokyo, Japan). FITC-OVA was prepared by reacting OVA (10 mg) with FITC (11.8 mg) in 0.5 M NaHCO₃ (4 mL, pH 9.0) at 4 °C for three days and subsequent dialysis [13 (link)]. 3-Methyl glutarylated dextrans (MGlu65-Dex and MGlu67-Dex-C₁₀) were prepared as previously reported [15 (link)].

BLM (PubChem CID: 5360373; Bleo) was obtained from Nippon Kayaku (Tokyo, Japan). Egg yolk phosphatidylcholine (EYPC), N-[methoxy (polyethyleneglycol) 2000]-distearoyl phosphatidylethanolamine (PEG-PE), and CHexPG-PE, which is distearoyl phosphatidylethanolamine with 2-carboxycyclohexane-1-carboxylated poly(glycidol) groups, were obtained from NOF Co. (Tokyo, Japan). Pyranine and Triton X-100 were obtained from Tokyo Chemical Industries Ltd. (Tokyo, Japan). p-Xylene-bis-pyridinium bromide (DPX) was obtained from Sigma (St. Louis, MO, USA). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) was obtained from ThermoFisher (Waltham, MA, USA).