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2 protocols using 3 3 4 dihydroxyphenyl l alanine l dopa

1

Synthesis and Characterization of Silicone Polymer Composites

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2.1. Materials and chemicals. Silicone hollow cylinders (L:22 mm, ID: 4 mm, WT: 0.5 g) were cut from extruded silicone tubing (Nusil MED-4720) and carbon dioxide (N48) was supplied by Air Liquide Denmark (Copenhagen, Denmark). 2-hydroxyethyl methacrylate (97%, HEMA), poly (ethylene glycol) methyl ether acrylate Mn 480 (PEGMEA), butyl methacrylate (BMA), 2,3,3-Trimethylindolenin 98%, 2-hydroxy-5-nitrobenzaldehyde, acetonitrile, diethyl ether, dichloromethane (DCM), hexane, potassium hydroxide, triethylamine, methacryloyl chloride, 2-bromoethanol, 98% ethylene glycol dimethacrylate (EGDMA), tetrahydrofuran (THF), doxycycline hyclate, curcumin, dopamine hydrochloride and ethanol (99.8%, EtOH) and 3-(3,4-Dihydroxyphenyl)-L-alanine (L-DOPA) were purchased from Sigma-Aldrich. Removal of the monomethyl ether hydroquinone (MEHQ) inhibitor from HEMA and PEGMEA and their purification was done as described previously 53 (link) . Diethyl peroxydicarbonate (DEPDC) in hexane was prepared as described by Xu et al 56 (link) and used as initiator for radical polymerization.
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2

Tyrosinase Inhibition and Antioxidant Assays

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Mangiferin, kojic acid, tyrosinase (tyrosinase from mushroom), 3-(3,4-Dihydroxyphenyl)-L-alanine (L-DOPA), phosphate buffer (0.175 mM, pH 6.8), phosphoric acid were sourced from Sigma Chemical Co., (St. Louis, MO, USA), and TRIS-HCl, HCl (77 mM), NaCl, CaCl2, acetate buffer (acetic acid 79 mM, sodium acetate 24 mM, pH 3.75) for enzymatic assay, and acetate buffer 0.3 M, pH 3.6 for FRAP assay were sourced from Avantor Performance Materials Poland S. A. FeCl3 × 6H2O, HPLC-grade methanol, and ethanol were purchased from P.O.Ch. (Gliwice, Poland).
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