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Luminol

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Luminol is a chemical compound used in analytical chemistry and forensic science. It is a light-emitting compound that can be used to detect the presence of trace amounts of blood or other oxidizing substances. Luminol reacts with these substances to produce a chemiluminescent reaction, which can be observed and photographed to aid in the identification and analysis of evidence.

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Lab products found in correlation

Isoluminol, by Merck Group (2 mentions) Superoxide dismutase, by Solarbio (2 mentions) Synergy h1 multi mode reader, by Agilent Technologies (2 mentions) Isoluminol, by Agilent Technologies (2 mentions) Krg ca 2 containing cell permeable luminol, by Merck Group (2 mentions) Wkymvm peptide, by Merck Group (2 mentions) Formyl methionyl leucyl phenylalanine (fmlp), by Merck Group (2 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (2 mentions) Luminescence reader, by Agilent Technologies (1 mentions) Lucigenin, by Merck Group (1 mentions) Luminol, by Merck Group (1 mentions)

3 protocols using luminol

1

Measuring NADPH Oxidase Activity via Chemiluminescence

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NADPH oxidase activity was measured by luminol/isoluminol-enhanced chemiluminescence (CL) using a microplate reader (Synergy H1 MultiMode Reader, BioTek, U.S.A) [30] . For intracellular ROS measurement, granulocytes were added to KRG-Ca 2+ containing cell permeable luminol (10 µg/mL; Sigma-Aldrich) and 50 U/mL superoxide dismutase (Solarbio) to scavenge extracellular superoxide, while extracellular ROS was measured in the presence of 10 µg/mL isoluminol (Sigma-Aldrich). Neutrophils and reagents were then incubated for 5 min at 37°C before stimulation with fMLP (1 µM; Sigma-Aldrich), WKYMVM peptide (1 µM; Sigma-Aldrich), or phorbol 12-myristate 13-acetate (PMA) (500 nM; Sigma-Aldrich). CL was recorded continuously for 20-25 min.
Zhang L., Chen Z., Li W., Liu Q., Wang Y., Chen X., Tian Z., Yang Q., An Y., Zhang Z., Mao H., Tang X., Lv G, & Zhao X. (2022). Combined Immunodeficiency Caused by a Novel De Novo Gain-of-Function RAC2 Mutation. Journal of clinical immunology, 42(6).
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2

Quantifying Reactive Oxygen Species in Rat Retina

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The chemilumigenic probes, luminol (5‐amino‐2,3‐dihydro‐1,4‐phthalazinedione, Sigma) and lucigenin (bis‐N‐methylacridiniumnitrate, Sigma), were used to assess the ROS level in rat retina.30 Briefly, retinas were isolated at days 3, 7 and 14 after euthanasia and then balanced with 1 × PBS. Samples were then placed into vials containing PBS‐HEPES buffer (pH 7.2). The level of ROS was analysed by supplementing lucigenin and luminol with a final concentration of 0.2 mM. The luminescent intensity was recorded at every 1 minutes through luminescence reader (BioTek, USA).
Mu H., Wang Y., Wei H., Lu H., Feng Z., Yu H., Xing Y, & Wang H. (2018). Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury. Journal of Cellular and Molecular Medicine, 22(10), 5008-5019.
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3

Measuring NADPH Oxidase Activity via Chemiluminescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
NADPH oxidase activity was measured by luminol/isoluminol-enhanced chemiluminescence (CL) using a microplate reader (Synergy H1 MultiMode Reader, BioTek, U.S.A) [30] . For intracellular ROS measurement, granulocytes were added to KRG-Ca 2+ containing cell permeable luminol (10 µg/mL; Sigma-Aldrich) and 50 U/mL superoxide dismutase (Solarbio) to scavenge extracellular superoxide, while extracellular ROS was measured in the presence of 10 µg/mL isoluminol (Sigma-Aldrich). Neutrophils and reagents were then incubated for 5 min at 37°C before stimulation with fMLP (1 µM; Sigma-Aldrich), WKYMVM peptide (1 µM; Sigma-Aldrich), or phorbol 12-myristate 13-acetate (PMA) (500 nM; Sigma-Aldrich). CL was recorded continuously for 20-25 min.
Zhao X., Zhang L., Chen Z., Li W., Liu Q., Wang Y., Chen X., Tian Z., Yang Q., An Y., Zhang Z., Mao H., Tang X., & Lv G. (2022). Combined Immunodeficiency Caused by a Novel De Novo Gain-of-Function RAC2 Mutation.
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