Proteome profiler mouse cytokine array a

The Proteome Profiler Mouse Cytokine Array A, (ARY006, R&D Systems) was used to quantify the 40 mouse proteins (cytokines, chemokines, and growth factors) from serum collected the day before surgery, and four hours after the 2^nd injection from mice injected with PBS, unmodified MSCs, and ZipperCells. Serum was diluted and mixed with a cocktail of biotinylated detection antibodies, according to the manufacturer’s instructions. The sample/antibody mixture was then incubated with the array membrane overnight at 4°C. The membranes were washed and incubated with streptavidin–horseradish peroxidase followed by chemiluminescent detection. The array data were quantitated to generate a protein profile and the results are presented as the average signal (pixel density) of the pairs of duplicate spots representing each cytokine or chemokine analyzed using MATLAB. The data presented are from three biological samples per group.

The Proteome Profiler Mouse Cytokine Array A, (ARY006, R&D Systems) was used to quantify the 40 mouse proteins (cytokines, chemokines, and growth factors) from serum collected the day before surgery, and four hours after the 2^nd injection from mice injected with PBS, unmodified MSCs, and ZipperCells. Serum was diluted and mixed with a cocktail of biotinylated detection antibodies, according to the manufacturer’s instructions. The sample/antibody mixture was then incubated with the array membrane overnight at 4°C. The membranes were washed and incubated with streptavidin–horseradish peroxidase followed by chemiluminescent detection. The array data were quantitated to generate a protein profile and the results are presented as the average signal (pixel density) of the pairs of duplicate spots representing each cytokine or chemokine analyzed using MATLAB. The data presented are from three biological samples per group.