Myosin vb

Manufactured by Novus Biologicals

Sourced in United Kingdom

Myosin Vb is a member of the myosin protein family. It functions as a molecular motor, responsible for intracellular transport and vesicle trafficking within eukaryotic cells.

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Lab products found in correlation

Fluorescently labeled phalloidin, by Thermo Fisher Scientific (1 mentions) Ezrin, by Santa Cruz Biotechnology (1 mentions) Syntaxin 3, by Abcam (1 mentions) C myc, by Takara Bio (1 mentions) Rab11a, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Dexamethasone (dex), by Bio-Techne (1 mentions) Ndrg1, by Cell Signaling Technology (1 mentions) Amplex red glucose assay kit, by Thermo Fisher Scientific (1 mentions) Phosstop easypack, by Roche (1 mentions) Horseradish peroxidase conjugated secondary antibody, by BD (1 mentions) Anti sglt1, by Abcam (1 mentions) Tamoxifen, by Merck Group (1 mentions)

2 protocols using myosin vb

Immunoblotting Antibodies Catalogue Reference

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Antibodies used in this study were ABCC2/ MRP2 (catalogue number MAB4150; Millipore, Burlington, MA), RAB11A (mouse) (catalogue number 610656; BioScience, Franklin Lakes, NJ), RAB11A (rabbit) (catalogue number ab128913; Abcam, Cambridge, MA), myosin Vb (catalogue number NBP1-87746; Novus), FLAG (catalogue number F3165; Sigma-Aldrich, St Louis, MO), UNC45A (rabbit) (catalogue number HPA039228; Merck, Kenilworth, NJ), UNC45A (mouse) (catalogue number ADI-SRA-1800-F; Enzo Life Sciences, Farmington, NY), beta-tubulin (catalogue number T4026; Merck), c-myc (catalogue number 631206; Takara, Kusatsu, Japan), ezrin (catalogue number SC58758; Santa Cruz Biotechnology, Dallas, TX), beta-actin (catalogue number A5441; Sigma-Aldrich), syntaxin-3 (catalogue number ab133750; Abcam), and munc18-2 (catalogue number ab103976; Abcam). Fluorescently labeled phalloidin (catalogue number A22284) was from Invitrogen.

Li Q., Zhou Z., Sun Y., Sun C., Klappe K, & van IJzendoorn S.C. (2022). A Functional Relationship Between UNC45A and MYO5B Connects Two Rare Diseases With Shared Enteropathy. Cellular and Molecular Gastroenterology and Hepatology, 14(2), 295-310.

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Immunoblot Analysis of Cellular Signaling

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The protease inhibitors used for lysate preparation for the immunoblots, PhosSTOP EASYpack (#04906837001), and complete EDTA-free (#11873580001) were purchased from Roche. Dexamethasone (#1126) was purchased from Tocris Bioscience. Antibodies against phospho-NDRG1^Thr346 (#5482), NDRG1 (#5196), phospho-PDK1^Thr346 (#3061), PDK1 (#3062), and GAPDH (#8884) were purchased from Cell Signaling. Antibodies against Phospho-PI3K p85^Tyr458/p55^Tyr199 (#PA5-17387), GLUT2 (#PA5-77459), Phospho-SGK1^Thr256 (#44-1260G), and Amplex™ Red Glucose Assay kit (#A22189) were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Additional antibodies: CFTR (AME4991, affinity purified polyclonal antibody raised against rat CFTR-produced by Dr. Ameen), NHE3-3H3 (#MABN1813) and SGK1 (#07-315) from EMD Millipore (Burlington, MA, USA), Phospho-PKCι^Thr563 (iota; #GTX25813) from GeneTex (San Antonio, TX, USA), Anti-SGLT1 (#ab14686) from Abcam (Cambridge, UK), and Myosin VB (#NBP1-87746) from Novus Biologicals (Centennial, CO, USA). Horseradish peroxidase-conjugated secondary antibodies were purchased from BD Bioscience (San Jose, CA, USA). Other reagents used in this study including tamoxifen (#T9262) were purchased from Sigma Aldrich (Burlington, MA, USA).

Ahsan M.K., dos Reis D.C., Barbieri A., Sumigray K.D., Nottoli T., Salas P.J, & Ameen N.A. (2022). Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID). Journal of Clinical Medicine, 11(14), 4179.

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