J 810 j 815 spectropolarimeter

Specific rotations were measured by an Autopol III automatic polarimeter (Rudolph Research Co., Ltd.). IR spectra were recorded on a Thermo Nicolet Nexus 470 FT-IR spectrometer. ECD spectra were measured on a JASCO J-810/J-815 spectropolarimeter. ¹H and ¹³C NMR spectra were recorded on a Bruker Avance-400FT NMR spectrometer. HRESIMS spectra were obtained on a Bruker APEX IV 70 eV FT-MS spectrometer and on a Thermo DFS spectrometer using a matrix of 3-nitrobenzyl alcohol. HF₂₅₄ silica gel for TLC was obtained from Qingdao Marine Chemistry Co. Ltd. Sephadex LH-20 (18–110 μm) was purchased from Pharmacia. The HPLC fingerprint chromatogram was prepared by HPLC-DAD Waters e2695 Separations module, Waters 2998 Photodiode Array Detector, and Waters Symmetry C₁₈ (5 μm 4.6 × 250 mm). Semi-preparative HPLC was performed on an Alltech 426 pump using UV detector, and the Prevail C₁₈ column (5 μm) was used for separation. Human liver tumor cell lines (HepG2, Sk-hep1, Hep3B, SUN475, SUN449) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA), and the cell lines SMMC-7721 and Huh-7 were purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China).

Specific rotations were measured by an Autopol III automatic polarimeter (Rudolph Research Co., Ltd.). IR spectra were recorded on a Thermo Nicolet Nexus 470 FT-IR spectrometer. UV spectra were measured on a Cary 300 spectrometer. ECD spectra were measured on a JASCO J-810/J-815 spectropolarimeter. The ¹H and ¹³C NMR spectra were recorded on a Bruker Avance-400FT NMR spectrometer. HRESIMS spectra were obtained on a Waters Xevo G2 Q-TOF spectrometer fitted with an ESI source. HF₂₅₄ silica gel for TLC was obtained from Qingdao Marine Chemistry Co. Ltd. Sephadex LH-20 was purchased from Pharmacia. Semi-preparative HPLC was performed on an Alltech 426 pump using UV detector, and the Prevail C₁₈ column (semipreparative, 5 μm) was used for separation.