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Ant hg

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Ant-hg is a lab equipment product designed for use in scientific research. It serves as a tool for detection and analysis of human IgG antibodies. The core function of Ant-hg is to enable researchers to identify and quantify human IgG levels in various sample types.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Polybrene, by Merck Group (2 mentions) Ant bl, by InvivoGen (2 mentions) Ant pr, by InvivoGen (2 mentions)

Spelling variants of this product

Ant-hg-1 (3 citations) Hygromycin B (ant-hg-1, (2 citations)

2 protocols using ant hg

1

Lentiviral Transduction and RNAi Knockdown

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All shRNA experiments were carried out by stable lentiviral transduction. Lentiviruses were generated by transfection of HEK293T cells with LeGO-iG2 or pInducer together with packaging plasmids psPAX.2 and pMD.2G. shRNA sequences for pInducer were from [60 (link)]. Plasmids psPAX.2 and pMD2.G were a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID: Addgene_12260 and Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID: Addgene_12259). LeGO-iG2 was a gift from Boris Fehse (Addgene plasmid # 27341; http://n2t.net/addgene:27341; RRID:Addgene_27341) [61 (link)]. shRNA sequences are listed in Supplementary Table 5. Infections were carried out using 8 μg/ml polybrene (#107689, Sigma). Two days after infection, cells were selected with 5 μg/ml blasticidin (#ant-bl, Invivogen), 2 μg/ml puromycin (#ant-pr, Invivogen) or 600 μg/ml hygromycin (#ant-hg, Invivogen), and pools of selected cells were used for downstream analyses. For transient depletion experiments, cells were transfected with siRNAs (Dharmacon) using Lipofectamine RNAiMAX (#13778030, Thermo Fisher Scientific) according to the manufacturer´s instructions. siRNA sequences are listed in Supplementary Table 6.
Schmidt S., Gay D., Uthe F.W., Denk S., Paauwe M., Matthes N., Diefenbacher M.E., Bryson S., Warrander F.C., Erhard F., Ade C.P., Baluapuri A., Walz S., Jackstadt R., Ford C., Vlachogiannis G., Valeri N., Otto C., Schülein-Völk C., Maurus K., Schmitz W., Knight J.R., Wolf E., Strathdee D., Schulze A., Germer C.T., Rosenwald A., Sansom O.J., Eilers M, & Wiegering A. (2019). A MYC/GCN2/eIF2α negative feedback loop limits protein synthesis to prevent MYC-dependent apoptosis in colorectal cancer. Nature cell biology, 21(11), 1413-1424.
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2

Lentiviral Transduction and RNAi Knockdown

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All shRNA experiments were carried out by stable lentiviral transduction. Lentiviruses were generated by transfection of HEK293T cells with LeGO-iG2 or pInducer together with packaging plasmids psPAX.2 and pMD.2G. shRNA sequences for pInducer were from [60 (link)]. Plasmids psPAX.2 and pMD2.G were a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID: Addgene_12260 and Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID: Addgene_12259). LeGO-iG2 was a gift from Boris Fehse (Addgene plasmid # 27341; http://n2t.net/addgene:27341; RRID:Addgene_27341) [61 (link)]. shRNA sequences are listed in Supplementary Table 5. Infections were carried out using 8 μg/ml polybrene (#107689, Sigma). Two days after infection, cells were selected with 5 μg/ml blasticidin (#ant-bl, Invivogen), 2 μg/ml puromycin (#ant-pr, Invivogen) or 600 μg/ml hygromycin (#ant-hg, Invivogen), and pools of selected cells were used for downstream analyses. For transient depletion experiments, cells were transfected with siRNAs (Dharmacon) using Lipofectamine RNAiMAX (#13778030, Thermo Fisher Scientific) according to the manufacturer´s instructions. siRNA sequences are listed in Supplementary Table 6.
Schmidt S., Gay D., Uthe F.W., Denk S., Paauwe M., Matthes N., Diefenbacher M.E., Bryson S., Warrander F.C., Erhard F., Ade C.P., Baluapuri A., Walz S., Jackstadt R., Ford C., Vlachogiannis G., Valeri N., Otto C., Schülein-Völk C., Maurus K., Schmitz W., Knight J.R., Wolf E., Strathdee D., Schulze A., Germer C.T., Rosenwald A., Sansom O.J., Eilers M, & Wiegering A. (2019). A MYC/GCN2/eIF2α negative feedback loop limits protein synthesis to prevent MYC-dependent apoptosis in colorectal cancer. Nature cell biology, 21(11), 1413-1424.
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