Products were analyzed by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) on a ICS3000 Dionex system (Dionex Corp., CA) consisting of a SP gradient pump, an autosampler (model AS-HV) and an electrochemical detector with a gold working electrode and Ag/AgCl as reference "Selective synthesis of galactooligosaccharides…"
[POSTPRINT] Digital CSIC 8 electrode. All eluents were degassed by flushing with helium. A pellicular anion-exchange 4 x 250 mm Carbo-Pack PA-1 column (Dionex) connected to a 4 x 50 mm CarboPac PA-1 guard column was used at 30 °C. Eluents were prepared with Milli-Q water and 50% (w/v) NaOH (Sigma-Aldrich). The initial mobile phase was 15 mM NaOH at 1.0 mL/min for 12 min. A mobile phase gradient from 15 to 200 mM NaOH was performed in 15 min at 1.0 mL/min, and it was maintained for 25 min. The peaks were analyzed by using Chromeleon software. The identification of the different carbohydrates was performed on the basis of commercially available standards or products purified in the laboratory. For those compounds whose standards were not available at sufficient amount, quantification was done based on the calibration curve of standards with the same degree of polymerization.
[POSTPRINT] Digital CSIC 8 electrode. All eluents were degassed by flushing with helium. A pellicular anion-exchange 4 x 250 mm Carbo-Pack PA-1 column (Dionex) connected to a 4 x 50 mm CarboPac PA-1 guard column was used at 30 °C. Eluents were prepared with Milli-Q water and 50% (w/v) NaOH (Sigma-Aldrich). The initial mobile phase was 15 mM NaOH at 1.0 mL/min for 12 min. A mobile phase gradient from 15 to 200 mM NaOH was performed in 15 min at 1.0 mL/min, and it was maintained for 25 min. The peaks were analyzed by using Chromeleon software. The identification of the different carbohydrates was performed on the basis of commercially available standards or products purified in the laboratory. For those compounds whose standards were not available at sufficient amount, quantification was done based on the calibration curve of standards with the same degree of polymerization.