Nikkor af s micro 60 mm f 2.8 g ed

Seedlings were grown from non-primed and primed seeds sown on top of fi lter paper soaked with water (0 dS m-1 -control) or 15 ml of saline solutions (2, 4, 6, and 8 dS m -1 , i.e. normal seedlings) incubated at 35 ºC and 12/12 h photoperiod. To measure root length, pictures were taken of ten day old seedlings using a digital camera (Nikon D80 with Nikkor AF-S Micro 60 mm f/2.8 G ED, Nikon), after which the seedlings were separated in shoots, roots and fresh and dry weight were determined. Dry weight was measured after placing shoots and roots at 104 °C for 24 h. The seedlings were grown randomized with 4 replicates of 25 seedlings per treatment and the data was statistically analyzed using the SISVAR software (Ferreira 2011).

After the mechanical tests, digital images of fractured specimens were captured by a digital camera (D800E, Nikon, Japan) equipped with a micro lens (Nikkor AF-S Micro 60 mm f/2.8G ED, Nikon, Japan). The specimens were sputter-coated with gold and analysed using a scanning electron microscope (JCM-6000 Plus, JEOL, Japan) in secondary electron mode with acceleration voltage of 15 kV.