Horseradish peroxidase hrp conjugated goat anti rabbit igg

The following polyclonal (pAb) and monoclonal (mAb) primary antibodies used in the study were obtained from the indicated commercial sources: BAF155 rabbit pAb (1:20; Santa Cruz), BAF155 mouse mAb (1:100; Santa Cruz), BAF170 rabbit pAb (1:100; Bethyl), BAF170 rabbit pAb (1:100; Sigma), Olig2 rabbit pAb (1:200; Millipore), PDGFRα rat pAb (1:200; BD Bioscience), Sox9 rabbit pAb (1:100; Millipore), Sox10 Guinea pig pAb (1:100; a gift from Prof. Michael Wegner), Ki67 rabbit pAb (1:50; Novocastra), Ki67 mouse mAb (1:100; Novocastra), IdU mouse mAb (1:50; Becton Dickinson), Casp3 rabbit pAb (1:100; Cell Signaling).
Secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:10,000; Covance), HRP-conjugated goat anti-mouse IgG (1:5,000; Covance), HRP-conjugated goat anti-rat IgG (1:10,000; Covance), and Alexa 488-, Alexa 568-, Alexa 594- and Alexa 647-conjugated IgG (various species, 1:400; Molecular Probes).

The following are the polyclonal (pAb) and monoclonal (mAb) primary antibodies used in this study: Pax6 rabbit pAb (1:200; Covance, Schwerte, Germany), H3K9Ac rabbit pAb (Abcam, Cambridge, UK), Sox2 goat (1:100; Santa Cruz, Dallas, TX, USA), Sox2 mouse mAb (1:100; R&D Systems, Minneapolis, MN, USA), Pax6 mouse mAb (1:100; Developmental Studies Hybridoma Bank, MA, USA), Tbr2 rabbit pAb (1:200; Abcam), H3ac rabbit pAb (Upstate, MA, USA), TNC rabbit pAb (Abcam), AP2γ mouse mAb (1:100; Abcam), PTPRZ1 rabbit pAb (Sigma, St. Louis, MO, USA), BAF155 mouse mAb (1:100; Santa Cruz), BAF155 rabbit pAb (1:20; Santa Cruz), Tbr1 rabbit pAb (1:300; Chemicon, St. Louis, MO, USA), HuCD mouse mAb (1:20; Invitrogen, Schwerte, Germany), Ctip2 rat pAb (1:200; Abcam), Cux1 rabbit pAb (1:100; Santa Cruz), Satb2 mouse mAb (1:200; Abcam), NeuN mouse mAb (Chemicon), Nestin mouse mAb (BD, Heidelberg, Germany), and Kat2a rabbit pAb (Abcam).
Secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:10,000; Covance), HRP-conjugated goat anti-mouse IgG (1:5000; Covance), HRP-conjugated goat anti-rat IgG (1:10,000; Covance), and Alexa 488-, Alexa 568-, Alexa 594-, and Alexa 647-conjugated IgG (various species, 1:400; Molecular Probes, Schwerte, Germany). Antibodies were purchased from the indicated manufacturers in Germany.