Two blood samples, 24 h postpartum and at the end of the experiment (pre-prandial, 08:00 h), were collected (5 mL/lamb) by venopunction of the jugular vein. The first sample was collected in tubes with anticoagulant (BD Vacutainer, Cuautitlan, State of Mexico, Mexico; K2 ethylenediaminetetraacetic acid) for hematological evaluations, and the second was deposited in tubes without anticoagulant (BD Vacutainer, Mexico; Serum) for separating the serum. Both samples were placed immediately in refrigeration (4°C) until analysis. Determination or erythrocytes, hematocrit, hemoglobin and leucocyte differential was carried out in an automatized hematological analyzer (Sysmex XS-1000i, Kobe, Japan).
Xs 1000i
Manufactured by Sysmex
Sourced in Japan, United States, Germany, United Kingdom
The XS-1000i is a hematology analyzer designed for use in clinical laboratories. It is capable of performing complete blood count (CBC) analysis, including red blood cell, white blood cell, and platelet measurement. The XS-1000i utilizes advanced technologies to provide accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Cobas c501 analyzer, by Roche (3 mentions)
Xe 2100, by Sysmex (2 mentions)
Cell dyn sapphire, by Abbott (2 mentions)
Ge vivid e7, by GE Healthcare (2 mentions)
Cellpack, by Sysmex (2 mentions)
Compact 2 type c, by Vitalograph (2 mentions)
Hla dr apc clone immu357, by Beckman Coulter (1 mentions)
Navios flow cytometer, by Beckman Coulter (1 mentions)
Synchron system, by Beckman Coulter (1 mentions)
Vitros 250 analyzer, by Johnson & Johnson (1 mentions)
Serum separator tube, by BD (1 mentions)
Cobas 6000, by Roche (1 mentions)
Dimension rxl max analyzer, by Siemens (1 mentions)
K3edta, by BD (1 mentions)
Sst tube, by BD (1 mentions)
Bactec peds plustm f, by BD (1 mentions)
Advia centaur xp immunoassay system, by Siemens (1 mentions)
Anti cd4 pe, by Miltenyi Biotec (1 mentions)
Anti cd31 apc, by BD (1 mentions)
Anti cd8 pe, by BD (1 mentions)
70 protocols using xs 1000i
1
Postpartum Blood Sample Analysis
2
Automated Blood Cell Counting with Sysmex XS-1000i
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Automated haematology analyzer (SYSMEX XS-1000i; Sysmex Corporation, Kobe, Japan) was used for the complete blood cell counts.
3
Full Blood Count Analysis Protocol
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Blood samples were collected in EDTA tubes for full blood counts using a Sysmex XS1000i (Sysmex Corporation, Kobe, Japan) according to manufacturer’s instructions.
4
Cord Blood Unit Processing for UCB-PL
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All cord blood units used in this study were collected by specialized midwives after a signed informed consent form was obtained by the mothers before gestation. The cord blood units were processed immediately after the reception at the Hellenic Cord Blood Bank (HCBB) of the Biomedical Research Foundation Academy of Athens (BRFAA), while the time between the collection and the processing did not exceed 48 h. A 0.5 mL blood sample was collected from each cord blood unit and was evaluated in a hematological analyzer (Sysmex XS1000i, Sysmex Europe, Norderstedt, Germany). If the cord blood unit did not fulfill the criteria for processing, cryopreservation and release outlined by FaCT-NetCORD [18 (link)], then it was used for UCB-PL production.
5
Differential Blood Cell Analysis
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For the study, differential blood counts were conducted using the Sysmex XS-1000i (Sysmex Corporation, Kobe, Japan). Additionally, high-sensitive CRP levels (with a detection limit of 0.20 mg/L) were measured using the SYNCHRON® System (Beckman Coulter, Fullerton, CA, USA) following the manufacturer’s instructions. Furthermore, a flow cytometric analysis of monocytes and polymorphonuclear neutrophils (PMN) for CD169-PE (clone 7-239), HLA-DR-APC (clone Immu357), and CD64-PB (clone 22) (all Beckman Coulter, three marker combination, C63854) was performed on the Navios® Flow Cytometer (Beckman Coulter). The relative median fluorescence intensity (MFI) for both monocytes and PMN were determined for all three antigens. Lymphocytes served as the negative population.
6
Platelet-Lymphocyte Ratio in SCAD
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For the purpose of this study, we examined a computer database to identify patients with SCAD referred to the Silesian Center for Heart Diseases in Poland who underwent coronary angiography and stent implantation between July 2006 and December 2011. In this database, information on coronary intervention, concomitant diseases, demographic data and laboratory parameters such as platelet and leukocyte counts are stored. The complete blood counts, which included total white blood cells, neutrophils, lymphocytes and platelets, were obtained using an automated blood counter Sysmex XS1000i and XE2100 (Sysmex Corporation, Kobe, Japan). Platelet-to-lymphocyte ratio was calculated as the ratio of the platelets to lymphocytes, obtained from the blood samples that were taken at the fasting state.
Patients undergoing hybrid revascularization, patients after orthotropic heart transplant, patients with known hematological diseases, patients on dialysis, or with other diseases limiting survival were excluded from the analysis. One patient died during the in-hospital period due to periprocedural complications. This patient was also excluded from this analysis.
The study was approved by the Local Ethics Committee at the District Chamber of Physicians.
Patients undergoing hybrid revascularization, patients after orthotropic heart transplant, patients with known hematological diseases, patients on dialysis, or with other diseases limiting survival were excluded from the analysis. One patient died during the in-hospital period due to periprocedural complications. This patient was also excluded from this analysis.
The study was approved by the Local Ethics Committee at the District Chamber of Physicians.
7
Hematological and Biochemical Analysis of Sickle Cell Disease
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Five ml of venous blood sample was drawn from each study participant into an EDTA tube, used to determine hematologic parameters. Hematologic parameters were performed using an automate Sysmex XS—1000 i (Lincolnshire, USA).
Five ml of venous blood sample was drawn from each study participant into an EDTA tube, used to determine haemoglobin electrophoresis. Sickle cell screening was performed using semi-automated electrophoresis technique with the Hydrasis II apparatus (SEBIA, France). The electrophoresis technique separates hemoglobin in acid and alkaline agarose gel. SCA was diagnosed in presence of production of Hb S with no Hb-A. The concentrations were measured by an integrated densitometer.
For LDH assay, the samples were collected in dry tubes. The serum LDH assay was performed with a spectrophotometer at 340 ηm with Thermo GENESYS 10S Bio apparatus (USA). The kit was provided by Cypress diagnostics (Landrop-Belgium). The reference values at 30°C were 160–320 U/L.
All analysis was performed at Institut National de Recherche Biomédicale (INRB) at Kinshasa, the DRC.
Five ml of venous blood sample was drawn from each study participant into an EDTA tube, used to determine haemoglobin electrophoresis. Sickle cell screening was performed using semi-automated electrophoresis technique with the Hydrasis II apparatus (SEBIA, France). The electrophoresis technique separates hemoglobin in acid and alkaline agarose gel. SCA was diagnosed in presence of production of Hb S with no Hb-A. The concentrations were measured by an integrated densitometer.
For LDH assay, the samples were collected in dry tubes. The serum LDH assay was performed with a spectrophotometer at 340 ηm with Thermo GENESYS 10S Bio apparatus (USA). The kit was provided by Cypress diagnostics (Landrop-Belgium). The reference values at 30°C were 160–320 U/L.
All analysis was performed at Institut National de Recherche Biomédicale (INRB) at Kinshasa, the DRC.
8
Comprehensive Lipid and Inflammatory Profiling
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Total cholesterol (TC), HDL cholesterol, TG, CRP and creatinine were measured on the Vitros 250 Analyzer (Johnson & Johnson, Rochester, NY, USA) in serum samples. LDL cholesterol and very low-density lipoprotein (VLDL) cholesterol were calculated according to known formulas [21 (link)]. The routine hemogram was measured in whole blood samples using the hematology analyzer Sysmex XS-1000i (Sysmex Corporation, Kobe, Japan).
9
Red Blood Cell Volume Variability
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Blood samples were obtained on admission and processed immediately. Complete blood counts were performed using the Sysmex XS1000i and XE2100 apparatus (Sysmex Corporation, Kobe, Japan). RDW was defined as the quotient of standard deviation (SD) of red blood cell volume and its mean volume and is expressed as a percentage according to the following formula: RDW = (SD of red blood cell volume / mean cell volume) × 100. Higher RDW values reflect greater variations in red blood cell volume.
10
Hematological Parameters in Doha, Qatar
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The collected blood specimens were analyzed at Hamad General Center (the largest general hospital) in Doha, Qatar. CBC parameters were measured using Cell-Dyn Sapphire (Abbott Laboratories, Diagnostic Division, Abbott Park, IL, USA) and Sysmex XS-1000i (Sysmex Corp., Kobe, Japan) hematology analyzers. Eleven hematology parameters investigated, which including red blood cells count, hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), white blood cells count (WBC), absolute neutrophil count (ANC), absolute lymphocyte count (LYMPH), absolute eosinophil count (EOS), absolute basophil count (BASO), absolute monocyte count (MONO), and platelet count (PLT). Anthropometric data, including weight and height, were accurately measured before blood collection; the Body Mass Index and height SDS (HtSDS were calculated for each adult.
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