Bgc 803

Nine human GC cell lines (SUN-216, BGC-823, AGS, BGC-803, NUGC4, MKN74, MKN45, SGC-7901, and HGC-27) and a non-malignant gastric mucosal epithelial cell line GES-1 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA). The human embryonic kidney (HEK) 293FT cell line was obtained from the American Type Culture Collection (Manassas VA, USA), and cultured and stored according to the provider’s instructions. All cell lines were maintained in a humidified cell incubator at 37 °C with an atmosphere of 5% CO₂.

Prostate cancer cell lines (DU145, C4-2B, PC3 and LNCaP), breast cancer cell lines (MCF-7, Bcap37, MDA-MB-435, MDA-MB-231), lung cancer line (A549), colon cancer lines (LoVo, HCT-116), liver cancer line (HepG-2) and gastric cancer cell lines (BGC-803, BGC-823, SGC-7901, MKN-28) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Prostate cancer cell lines, breast cancer cell lines, lung cancer line, colon cancer lines and liver cancer line were cultured in DMEM medium. BGC-823 cells were grown in RPMI 1640 medium. DMEM and RPMI 1640 media were provided by American Thermal Sciences Corporation, and 10% (v/v) fetal bovine serum, 100 mg streptomycin/mL and 100 U penicillin/mL were added. All cells were incubated in humidified air at 37 ℃ and 5% CO₂.

The cell lines SGC-7901, BGC-803, HGC-27, AGS, SNU-719, and HEK293, as well as NK-92, were acquired from the Cell Bank of the Chinese Academy of Sciences in Shanghai, China. The cells were grown in medium with 10% fetal bovine serum (FBS), which was maintained at 37 °C with 5% CO2.