Interferon ifn γ

Recombinant granulocyte-macrophage colony stimulating factor (GM-CSF), macrophage (M)-CSF, interleukin (IL)-1β, IL-6, IL-4, IL-10, IL-13, interferon (IFN)-γ, lymphotoxin (LT)-α, tumor necrosis factor (TNF), M-CSF and GM-CSF were purchased from ProSpec-Tany Technogene (Rehovot, Israel), transforming growth factor (TGF)-β1 from R&D Systems (Minneapolis, MN), and dexamethasone was purchased from Sigma-Aldrich (St. Louis, MO). A mouse monoclonal antibody (clone 3C9) that recognizes the IgV domain of human CRIg was kindly provided by Dr. Menno van Lookeren Campagne (Genentech, San Francisco, CA). RPMI 1640 tissue culture medium, foetal calf serum (FCS) and L-glutamine were purchased from SAFC Biosciences (Lenexa, KS).

The impact of an inflammatory environment on SKP is evaluated as previously described [33 (link)]. Briefly, SKP are stimulated for 18 h using a cocktail of pro-inflammatory cytokines: 25 ng/mL interleukin (IL)-1β (Peprotech, Rocky Hill, USA), 1 × 10³ U/mL interferon (IFN)-γ, 50 ng/mL tumor necrosis factor alpha (TNFα), and 3 × 10³ U/mL IFN-α (all from Prospec Inc, Rehovot, Israel).