The reactions were performed using a Loopamp DNA Amplification Reagent Kit (Eiken Chemical). Detection of the LAMP amplicons was performed by real-time measurements of turbidity using a Loopamp real-time turbidimeter LA-200 (Eiken Chemical) and visual observations of the colour changes with the naked eye under natural light. The final reaction volume was 25 μL, comprising 40 pmol of the FIP primer and 40 pmol of the BIP primer, 20 pmol of each loop primer, 5 pmol of F3 and B3 primers, 1 μL of the Bst DNA polymerase, reaction buffer (20 mM Tris–HCl, 10 mM KCl, 8 mM MgSO4, 10 mM (NH4)2SO4, 0.1% Tween 20, 0.8 M betaine, and 1.4 mM each dNTP), and 1 μL of plasmid DNA or 8 μL of genomic DNA samples. Reactions were carried out at 62, 63, 64, and 65 °C in duplicate to find the optimum temperature for the LAMP amplification.
Loopamp real time turbidimeter la 200
Manufactured by Eiken Chemical
The Loopamp real-time turbidimeter LA-200 is a laboratory instrument designed for real-time monitoring of turbidity in reaction mixtures. It measures the optical density of the sample to provide information about the turbidity levels during the reaction process.
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Lab products found in correlation
2 protocols using loopamp real time turbidimeter la 200
1
LAMP Amplification of Plasmid and Genomic DNA
2
RT-LAMP for SARS-CoV-2 Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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According to the manufacturer’s instructions, RT-LAMP was performed using the Loopamp SARS-CoV-2 Detection kit (Eiken Chemical Co., Ltd., Tokyo, Japan). The reaction volume of 25 μL contained 10 μL of purified RNA and 15 μL of reaction mix containing 172.3 ng/μL of SARS-CoV-2 specific primer sets. The mixture was incubated for 35 min at 62.5 °C, and the process was monitored using a Loopamp Real-time Turbidimeter (LA-200; Eiken Chemical). For visual evaluation of fluorescence, the reaction tube was illuminated with ultraviolet light using an ultraviolet illumination system (WSE-5300; ATTO, Tokyo, Japan) and observed by the naked eye.
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