Lung single-cell suspensions and BMDC suspensions collected from different groups were stained with PE anti-mouse CD11c (eBioscience, San Diego, CA, USA) and APC-Cy7 anti-mouse F4/80 (Biolegend Inc., San Diego, CA, USA). DCs were marked as CD11c+ and F4/80− cells. This was followed by analysis of the phenotype and the maturation of DCs using FITC anti-mouse CD80, FITC anti-mouse major histocompatibility complex II (MHCII), FITC anti-mouse CD40 (eBioscience, San Diego, CA, USA), and APC anti-mouse CD86 (Biolegend Inc., San Diego, CA, USA) stains. Among them, cDCs were marked as CD11c+MHCII+ double-positive cells. In addition, the cDCs2 (type 2 cDCs) in lung and spleen MNCs were stained as CD11c+CD11b+ double-positive cells using PE anti-mouse CD11c and APC anti-mouse CD11b (Biolegend Inc., San Diego, CA, USA). All the cells, after being washed with PBS, were analyzed using Flow Cytometer (FACSAria™ III, BD Biosciences, USA). The FlowJo software (FlowJo LLC, Ashland, Ore) was used to analyze the data.
Fitc anti mouse cd40
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC anti-mouse CD40 is a fluorescently labeled antibody that binds to the CD40 receptor on the surface of mouse cells. CD40 is a co-stimulatory molecule that plays a key role in immune cell activation and signaling. This product can be used for the identification and analysis of CD40-expressing cells in mouse samples using flow cytometry or other immunological techniques.
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Lab products found in correlation
Anti mouse cd11c pe, by Thermo Fisher Scientific (2 mentions)
Apc cy7 anti mouse f4 80, by BioLegend (2 mentions)
Fitc anti mouse cd80, by Thermo Fisher Scientific (2 mentions)
Pe anti mouse cd11c, by BioLegend (2 mentions)
Apc anti mouse cd11b, by BioLegend (2 mentions)
Fitc anti mouse major histocompatibility complex 2, by Thermo Fisher Scientific (2 mentions)
Apc anti mouse cd86, by BioLegend (2 mentions)
2 protocols using fitc anti mouse cd40
1
Identifying Dendritic Cell Subsets
2
Phenotypic Analysis of Dendritic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lung single cell suspensions and BMDCs suspensions collected from different groups were stained with PE anti-mouse CD11c (eBioscience, San Diego, CA, USA) and APC-Cy7 anti-mouse F4/80 (Biolegend Inc., San Diego, CA, USA). DCs were marked as CD11c + and F4/80 -cells. This was followed by analysis of the phenotype and the maturation of DCs using FITC anti-mouse CD80, FITC anti-mouse major histocompatibility complex II (MHCII), FITC anti-mouse CD40 (eBioscience, San Diego, CA, USA) and APC anti-mouse CD86 (Biolegend Inc., San Diego, CA, USA) stains. Among them, cDCs were marked as CD11c + MHCII + double positive cells. In addition, the cDCs2 (type 2 cDCs) in lung and spleen MNCs were stained as CD11c + CD11b + double positive cells using PE anti-mouse CD11c and APC anti-mouse CD11b (Biolegend Inc., San Diego, CA, USA). All the cells, after being washed with PBS, were analyzed using Flow Cytometer (FACSAria™ III, BD Biosciences, USA). The FlowJo software (FlowJo LLC, Ashland, Ore) was used to analyze the data.
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