For mitochondrial assays, NK-92 cells were stimulated with 1% CSE and 100 ng/ml PPD for another 24 h. NK-92 cells were then incubated with 50 nM Mito Tracker Green (MTG) (Beyotime Biotechnology Co., Ltd, Shanghai, China) and 12.5 nM Mito Tracker Deep Red (MTDR) (Invitrogen, Life Technologies, Carlsbad, CA, USA) in PBS for 30 min at 37°C prior to staining. The stained cells were measured through Beckman CytoFLEX S at 488/525 nm and 644/655 nm, respectively. Data were analyzed using FlowJo V10 software.
Mito tracker green mtg
Manufactured by Beyotime
Sourced in China
Mito Tracker Green (MTG) is a fluorescent dye used to specifically label mitochondria in live cells. It is a cell-permeant dye that accumulates in active mitochondria, allowing for the visualization and tracking of these organelles.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using mito tracker green mtg
1
Mitochondrial Assay in NK-92 Cells
2
Mitochondrial Tracker Fluorescence Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells following the above treatment were loaded fluorescence probe Mito-Tracker Green (MTG, Beyotime) for 20 min. After washing 3 times with PBS, the florescence intensities were observed under a confocal laser microscopy (LSM800).
3
Mitochondrial Mass and Membrane Potential Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mitochondrial-selective probe Mito Tracker Green (MTG, Beyotime) was used to detect the mitochondrial mass. This dye could localize to mitochondria regardless of Δψm. Cells were incubated with 100 nmol/L of MTG dye in the dark for 20 minutes at 37°C, and immediately analyzed with a BD FACSAria flow cytometer (BD Biosciences). Cells were also incubated with 10 μg/mL of JC-1 in the dark for 20 minutes at 37°C, and quantified Δψm with flow cytometry analysis. The JC-1 ratio was calculated as the ratio of red fluorescence to green fluorescence.
4
Oocyte Mitochondrial Dynamics Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The changes of the oocytes mitochondrial membrane potential (ΔΨm) were monitored by incubating in culture medium at 37 °C for 20 min with JC-1 (Beyotime, China). After fully washing, oocytes images were acquired using scanning microscope in selective channel for green and red (590 nm and 485 nm). The distribution of mitochondria in oocytes was labeled with Mito Tracker Green (MTG, Beyotime, China) in culture medium at 37 °C for 30 min, and measured by Laser scanning confocal microscopy.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!