Sliceomatic 4 3 and 5

As described previously, detailed body composition of all subjects was performed by using whole-body MRI with a 1.5T scanner (Magnetom Vision or Magnetom Avanto, Siemens Medical Systems, Erlangen, Germany).^{30 (link), 31 (link)} Briefly, participants were examined in a supine position with their arms extended above their heads and they were asked to hold their breath during measurement of abdominal and thoracic regions for image acquisition. In the present analysis volumes of SAT and VAT were manually segmented by using segmentation software (SliceOmatic 4.3 and 5.0, Tomovision, Montreal, Canada). SAT was analyzed from ankle to wrist. In this case SAT_legs were defined from ankle to femoral heads and SAT_arms were defined from humerus heads to wrist. Between femoral and humerus heads volume of SAT_trunk was assessed. VAT was defined as intra-abdominal fat between femoral heads and the top of the liver. Volumes of SAT and VAT were determined by the sum of all adipose tissue areas (cm²) multiplied by slice thickness. Volume data were then transformed into tissue masses by using the following density: 0.923 g cm⁻³ for SAT and VAT.