Human or mouse xenograft tumors were fixed in 10% neutral buffered formalin followed by routine paraffin embedding and processing for immunohistochemical staining (including deparaffinization, rehydration, and antigen retrieval in boiling 0.01M citric acid solution, pH 6.8). Vector M.O.M. fluorescein or peroxidase Immunodetection Kits (Vector Laboratories, CA) were used for detection with SigmaFast diaminobenzidene (DAB) as a peroxidase substrate. Nuclei were counterstained with hematoxylin.
Sigmafast diaminobenzidene
Manufactured by Vector Laboratories
SigmaFast diaminobenzidene is a chromogenic substrate used in immunohistochemistry and enzyme-linked immunosorbent assays (ELISA) to visualize the presence of target proteins or antigens. It produces a brown colored precipitate at the site of the enzyme-catalyzed reaction, allowing for the detection and localization of the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sigmafast diaminobenzidene
1
Immunohistochemical Staining of Xenograft Tumors
2
Immunohistochemical Staining of Xenograft Tumors
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