CNVs were generated as described previously.49 (link) In brief, under deep anesthesia, laser photocoagulation (532 nm, 180 mW, 75 μm, 100 ms; Novus Spectra; Lumenis, Tokyo, Japan) was performed around the optic disc using a slit-lamp delivery system with a cover glass as a contact lens. Four laser spots per eye for immunohistochemistry and six laser spots for mRNA and protein analysis were placed. The formation of a subretinal bubble at the time of laser photocoagulation indicated the rupture of Bruch’s membrane. (P)RR-PshRNA, aflibercept (2.5 μg; Bayer, Leverkusen, Germany), or a mixture of (P)RR-PshRNA and aflibercept in 1 μL PBS per eye was injected into the vitreous cavity of mice immediately after laser injury. An additional injection was given on day 7 for quantification of subretinal fibrosis at day 21 after laser. Controls received control-PshRNA or PBS. The dose of in vivo injection of control or (P)RR-PshRNA was determined to be 100 pM in 1 μL PBS per eye, at which (P)RR-PshRNA significantly suppresses the size of CNV in dose-ranging experiments.
Novus spectra
Manufactured by Lumenis
Sourced in Japan
The Novus Spectra is a laser system designed for various medical and aesthetic applications. It features a dual-wavelength configuration with a 532nm green laser and a 1064nm infrared laser.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using novus spectra
1
CNV Induction and Intervention Protocol
2
Laser-induced choroidal neovascularization model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The laser-induced CNV model was generated as described previously [20 (link)]. In brief, laser injury (532 nm, 180 mW, 75 μm, 100 ms, Novus Spectra; Lumenis, Tokyo, Japan) was conducted around the optic disc using a slit-lamp delivery system. Four laser spots per eye for quantification of CNV and choroidal fibrosis were performed. The formation of a subretinal bubble at the time of laser injury indicated the rupture of Bruch’s membrane. Immediately after laser injury, 1 μg of PDGFR-β neutralizing antibody (APB5, Thermo Fisher Scientific, Waltham, MA, USA) or isotype-matched IgG (Thermo Fisher Scientific) in 1 μL, was injected into the vitreous cavity of mice. An additional intravitreal injection was given to mice on day 7 for the quantification of subretinal fibrosis formation at 21 days after CNV induction.
3
Laser Photocoagulation of Rat Retina
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Laser photocoagulation was performed under general anesthesia as described before. Prior to laser pulse application, the pupils of the rats were dilated with focal administration of a 0.5% phenylephrine hydrochloride-0.5% tropicamide solution (Santen Pharmaceutical, Osaka, Japan). Lubricating eyedrops (Genteal; Alcon Laboratories, Fort Worth, TX, USA) placed on a glass cover slip were applied to the cornea, and the retina was viewed through a slit lamp microscope (NOVUS Spectra™; Lumenis, Tokyo, Japan).
While viewing the ocular fundus, five laser spots were created per eye around the optic disc. The laser parameters were set to the following: intensity, 200 mW; duration, 0.1 second; and spot diameter, 75 µm. The presence of a bubble at the time of the laser application was taken as an indication that Bruch’s membrane had been sufficiently ruptured.
While viewing the ocular fundus, five laser spots were created per eye around the optic disc. The laser parameters were set to the following: intensity, 200 mW; duration, 0.1 second; and spot diameter, 75 µm. The presence of a bubble at the time of the laser application was taken as an indication that Bruch’s membrane had been sufficiently ruptured.
4
Laser-Induced Choroidal Neovascularization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4 weeks after AAV injection, mice were used for CNV induction. Briefly, pupils were dilated with tropicamide eye drops (Santen Pharmaceutical), and mice were anesthetized with a mixture of zoletil (60 mg/kg) and xylazine (10 mg/kg). Laser photocoagulation was performed using NOVUS Spectra (LUMENIS). The laser parameters used in this study were: 532 nm wavelength, 50 ms exposure time, 200 mW power, and 50 μm spot size. 3 to 4 laser burns around the optic disc were induced for FFA detection. 20 laser burns around the optic disc were induced for ELISA detection. Mice with vitreous hemorrhage were excluded from the study. CNV and ELISA analysis was conducted 8 days after laser burn.
5
Laser-Induced Bruch's Membrane Rupture in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The animal experiments were conducted according to the Association for Research in Vision and Ophthalmology (ARVO) Statement on the Use of Animals in Ophthalmic and Vision research, and approved by the Animal Experimentation Ethics Committee of Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong (approval number: EC20121123(5)-P21). Two-month-old C57BL/6 mice were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd., China. All mice were housed under standard conditions at 22 ± 1°C, 40 ± 10% humidity, and with 12:12 hour dark-light cycle. Standard rodent chow and water were provided ad libitum. The mice were anesthetized with 100 mg/kg ketamine and 10 mg/kg xylazine, and the pupils were dilated with 0.5% tropicamide eyedrops. Bruch's membrane rupture was created using a 532-nm laser photocoagulator (NOVUS Spectra; Lumenis, San Jose, CA) with a slit lamp delivery system that was used to create 3 burns, which were located at 3 disc-diameters from the optic disc at 3, 6, and 9 o'clock with the power of 120 mW, spot size of 75 µm and duration of 0.1 seconds. Only burns with production of a bubble at the time of the laser were included, as it indicates the rupture of Bruch's membrane.
6
Laser-induced Retinal Degeneration in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six- to eight-week-old mice were anesthetized with 10% pentobarbital, and their pupils were dilated with 0.5% tropicamide and 0.5% phenylephrine eyedrops (Santen Pharmaceutical Co., Osaka, Japan). Laser photocoagulation was then performed at 8 spots per eye around the optic disk with a wavelength of 532 nm, a power of 200 milliwatt, a duration of 100 ms, and a spot size of 75 μm, using a slit lamp delivery system (Novus Spectra; Lumenis, Tokyo, Japan) as described previously (31 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!