Rat igg2b isotype antibody

Manufactured by BioXCell

The Rat IgG2b isotype antibody is a laboratory reagent used in various immunological applications. It is a type of immunoglobulin G (IgG) antibody that is specific to the IgG2b subclass found in rats. This antibody can be used as a tool for detecting and quantifying the presence of rat IgG2b in samples.

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Lab products found in correlation

Be0101, by BioXCell (1 mentions) Anti ifn γ apc, by BioLegend (1 mentions) Anti cd4 pacific blue, by BioLegend (1 mentions) Anti cd3 apc efluor780, by Thermo Fisher Scientific (1 mentions) Anti cd8α percp cy5, by BioLegend (1 mentions) Anti cd69 fitc, by BioLegend (1 mentions) Anti cd44 alexa fluor 700, by BioLegend (1 mentions) Anti cd45 apc efluor780, by BioLegend (1 mentions)

2 protocols using rat igg2b isotype antibody

Immunotherapy combinations in mice

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One week after intrasplenic injection, mice received (a) rat IgG2b isotype antibody (Bioxcell BE0090) and vehicle (0.5% HPMC/0.1% Tween-80) (n = 6), (b) ALK5i and isotype antibody (n = 6), (c) anti-PD-L1 antibody (Bioxcell BE0101) and vehicle (n = 6), or (d) combination of ALK5i and anti-PD-L1 antibody (n = 6). Anti-PD-L1 and isotype antibody were injected at 10 mg/kg intraperitoneal (IP) twice per week. After 3 weeks, mice were euthanized and tissues were collected for analysis.

Nair R., Lannagan T.R., Jackstadt R., Andrusaite A., Cole J., Boyne C., Nibbs R.J., Sansom O.J, & Milling S. (2024). Co-inhibition of TGF-β and PD-L1 pathways in a metastatic colorectal cancer mouse model triggers interferon responses, innate cells and T cells, alongside metabolic changes and tumor resistance. Oncoimmunology, 13(1), 2330194.

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Multiparameter Analysis of CD8+ T Cells

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₅₃₃IYSTVASSL₅₄₁ (IVL_533-541)-tetramer was bought from MBL (cat. NO.TS-M520-1), anti-CD8α depletion antibody (clone: YTS 169.4). Rat IgG2b isotype antibody (clone: LTF-2) was purchased from Bio X Cell. Antibodies for flow cytometry included anti-CD3-APC-eFluor780 (clone: 17A2; eBioscience), anti-CD4-Pacific Blue (clone: GK1.5; BioLegend), anti–CD8α-PerCP/Cy5.5 (clone: 53–6.7; BioLegend), anti-CD11a-PE/Cy7 (clone: 2D7; BD Bioscience), anti–CD44-Alexa Fluor 700 (clone: IM7; BioLegend), anti-CD45-APC-eFluor780 (clone:30-F11;Biolegend), anti-CD62L-eVolve 605 (clone: MEL-14; eBioscience), anti-CD127-PE & PE/Cy7 (clone: A7R34; BioLegend), anti-KLRG1-FITC & BV510 (clone: 2F1/KLRG1; BioLegend), anti-CD103-APC (clone: 2E7; BioLegend), anti-CD69-FITC (clone: H1.2F3; BioLegend) and anti-IFNγ-APC (clone: XMG1.2; BioLegend), anti-Eomes-PE & PE/Cy7 (clone: Dan11mag; eBioscience).

Zheng X., Oduro J.D., Boehme J.D., Borkner L., Ebensen T., Heise U., Gereke M., Pils M.C., Krmpotic A., Guzmán C.A., Bruder D, & Čičin-Šain L. (2019). Mucosal CD8+ T cell responses induced by an MCMV based vaccine vector confer protection against influenza challenge. PLoS Pathogens, 15(9), e1008036.

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