Anti-MST1 (Cell Signaling Technology, USA), anti-LATS1 (Cell Signaling Technology, USA), anti-YAP (Cell Signaling Technology, USA), anti-YAP (Santa Cruz, USA), anti-PP2A Bβ (Proteintech, China), anti-Myc (Proteintech, China), anti-FLAG (Proteintech, China), or anti-IgG (Santa Cruz, USA) antibodies were incubated with Protein A/G Magnetic Beads (Bimake, USA) for 4 h at 4°C. Samples were lysed with Thermo Scientific Pierce IP Lysis Buffer (Thermo Fisher Scientific, USA) with protease inhibitor mixture (Bimake, USA) and incubated with an antibody-bead complex overnight at 4°C. The immunoprecipitation products were then precipitated by the antibody-bead complex using a magnetic rack and analyzed by western blotting.
Thermo scientific pierce ip lysis buffer
Manufactured by Thermo Fisher Scientific
Sourced in United States
Thermo Scientific Pierce IP Lysis Buffer is a ready-to-use buffer designed for cell lysis and immunoprecipitation experiments. It contains a proprietary detergent formulation that efficiently solubilizes a wide range of proteins while maintaining their native structure and interactions.
Automatically generated - may contain errors
Lab products found in correlation
Protein a g magnetic beads, by Selleck Chemicals (1 mentions)
Anti mst1, by Cell Signaling Technology (1 mentions)
Anti flag, by Proteintech (1 mentions)
Protease inhibitor mixture, by Selleck Chemicals (1 mentions)
Anti myc, by Proteintech (1 mentions)
Anti lats1, by Cell Signaling Technology (1 mentions)
Anti yap, by Cell Signaling Technology (1 mentions)
Anti yap, by Santa Cruz Biotechnology (1 mentions)
2 protocols using thermo scientific pierce ip lysis buffer
1
Immunoprecipitation Assay for Protein Analysis
2
Identification of H19-Binding Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A biotin-coupled H19 probe (sense and antisense) was specially designed using Thermo Scientific Pierce™ RNA 3′ End Desthiobiotinylation Kit (Thermo Fisher Scientific, Waltham, MA, USA). Cell lysate of BGC-823 cells was prepared using Thermo Scientific Pierce IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA, USA). Incubated cell lysate with 50 pmol biotin-labeled probes and streptavidin magnetic beads for 1 hour. After eluting the protein, mass spectrometry was used to detect the proteins binding with H19.
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