Nociceptin

Manufactured by Cayman Chemical

Sourced in United States

Nociceptin is a laboratory product manufactured by Cayman Chemical. It is a neuropeptide that functions as a ligand for the opioid receptor-like 1 (ORL1) receptor. The core function of Nociceptin is to serve as a research tool for the study of the ORL1 receptor and its role in various physiological processes.

Automatically generated - may contain errors

Lab products found in correlation

Human il 1β, by Thermo Fisher Scientific (1 mentions) Pronase e, by Merck Group (1 mentions) Forskolin, by Bio-Techne (1 mentions) Collagenase p, by Roche (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Chondrocyte basal medium, by PromoCell (1 mentions) Chondrocyte growth medium supplementmix, by PromoCell (1 mentions) Cell culture materials, by Thermo Fisher Scientific (1 mentions) U46619, by Cayman Chemical (1 mentions) Gsk1120212, by Selleck Chemicals (1 mentions)

2 protocols using nociceptin

Chondrocyte Culture with Cytokines and Opioids

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Human IL-1β and human TNF were purchased from PeproTech (Rocky Hill, NJ, USA) and dissolved in water. Forskolin was purchased from Tocris Bioscience (Bristol, UK) and dissolved in dimethyl sulfoxide (DMSO)/water (final DMSO dilution 1:200). Β-Endorphin, Nociceptin and Dynorphin A were purchased from Cayman Chemical (Ann Arbor, MI, USA) and dissolved in water. Pronase E was obtained from Merck KGaA (Darmstadt, Germany), and collagenase P from Roche Diagnostics GmbH (Mannheim, Germany). The chondrocyte culture medium contains Chondrocyte Basal Medium + 10% Chondrocyte Growth Medium SupplementMix (both from PromoCell GmbH, Heidelberg, Germany) + 1% penicillin/streptomycin solution (Life Technologies Europe BV, NN Bleiswijk, The Netherlands). For all stimulation experiments, stock solutions of cytokines, Forskolin and opioid neuropeptides were diluted to the final concentration in chondrocyte culture medium.

Eitner A., Sparing S., Kohler F.C., Müller S., Hofmann G.O., Kamradt T., Schaible H.G, & Aurich M. (2022). Osteoarthritis-Induced Metabolic Alterations of Human Hip Chondrocytes. Biomedicines, 10(6), 1349.

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Cell culture and pharmacological assays

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Cell culture materials were from Invitrogen, U-46619 and nociceptin were from Cayman, GSK-1120212 (Trametinib) were from Selleckchem, and all other reagents were from Sigma Aldrich unless stated otherwise.

Schrage R., Schmitz A.L., Gaffal E., Annala S., Kehraus S., Wenzel D., Büllesbach K.M., Bald T., Inoue A., Shinjo Y., Galandrin S., Shridhar N., Hesse M., Grundmann M., Merten N., Charpentier T.H., Martz M., Butcher A.J., Slodczyk T., Armando S., Effern M., Namkung Y., Jenkins L., Horn V., Stößel A., Dargatz H., Tietze D., Imhof D., Galés C., Drewke C., Müller C.E., Hölzel M., Milligan G., Tobin A.B., Gomeza J., Dohlman H.G., Sondek J., Harden T.K., Bouvier M., Laporte S.A., Aoki J., Fleischmann B.K., Mohr K., König G.M., Tüting T, & Kostenis E. (2015). The experimental power of FR900359 to study Gq-regulated biological processes. Nature Communications, 6, 10156.

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