Avance drx 500

Manufactured by Bruker

Sourced in Germany, United States

The Avance DRX 500 is a nuclear magnetic resonance (NMR) spectrometer designed for advanced analytical applications. It features a 500 MHz superconducting magnet and provides high-resolution, multi-dimensional NMR capabilities for the structural elucidation of chemical compounds.

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Lab products found in correlation

Ir 460 spectrometer, by Shimadzu (3 mentions) Avance drx 400, by Bruker (3 mentions) Silica gel 60, by Merck Group (3 mentions) Avance dpx 300, by Bruker (3 mentions) Avance 3 600, by Bruker (3 mentions) Drx 300, by Bruker (2 mentions) Vxr 300, by Agilent Technologies (2 mentions) Avance 400, by Bruker (2 mentions) Silica gel, by Qingdao Marine Chemical (2 mentions) Am 300, by Bruker (2 mentions) Alliance 2695 separation module, by Waters Corporation (2 mentions) Pda 2996 uv detector, by Waters Corporation (2 mentions) Silica gel, by Merck Group (2 mentions) Avance 800 mhz, by Bruker (1 mentions) Spectrum one, by PerkinElmer (1 mentions) D max c 3, by Rigaku (1 mentions) Sonicator 3200, by Bandelin (1 mentions) 5973 mass spectrometer, by Shimadzu (1 mentions) Avance drx 300, by Bruker (1 mentions) 1100 lc msd, by Agilent Technologies (1 mentions)

Spelling variants of this product

Avance 500 (319 citations) DRX-500 (224 citations) Avance DRX-500 spectrometer (68 citations) Avance DRX 500 MHz (20 citations) Avance DRX (16 citations) Avance DRX-500 MHz spectrometer (7 citations) Avance DRX III 500 (5 citations) DRX 500-AVANCE FT-NMR instrument (2 citations) AVANCE Model DRX-500 (2 citations) Avance DRX 500 MHz NMR spectrometer (2 citations)

84 protocols using avance drx 500

Backbone Assignment and Ligand Binding of Swi6 CSD

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For backbone assignment of the Swi6 CSD, protein was expressed in M9 minimal media containing ¹⁵N-ammonium chloride and ¹³C-glucose as the sole nitrogen and carbon source, respectively. Proteins were purified as previously described (above) with exchange into a final buffer containing 20 mM Hepes pH 7.8, 150 mM KCl, and 2 mM DTT. Backbone assignments were obtained from nitrogen HSQC and triple-resonance (HNCA, CBCA(CO)NH) experiments recorded at 303K on either a Bruker Avance DRX500 or Bruker Avance 800 MHz spectrometer equipped with cryogenic probes. For binding experiments, unlabeled peptides were added to either 100 μM ¹⁵N-labeled Swi6 CSD or Chp2 His-tagged CSD and nitrogen HSQC spectra were recorded at 303K on a Bruker Avance DRX500 spectrometer. Chemical shift perturbations were calculated from the equation:

\sqrt{0.5 ({(δ H_{bound} - δ H_{free})}^{2} + {(0.2 (δ N_{bound} - δ N_{free}))}^{2})}

where the factor of 0.2 is used as a scaling factor for the nitrogen spectral width. Titration data were only fitted to obtain K_d values if the chemical shift perturbation between apo and final peptide concentration for a residue was greater than the mean plus one standard deviation.

Isaac R.S., Sanulli S., Tibble R., Hornsby M., Ravalin M., Craik C.S., Gross J.D, & Narlikar G.J. (2017). Biochemical basis for distinct roles of the heterochromatin proteins Swi6 and Chp2. Journal of molecular biology, 429(23), 3666-3677.

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NMR Spectroscopy Characterization Protocol

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NMR spectra were measured on a Bruker DRX 500 Avance (1H, 500.13 MHz and 13C, 125.77 MHz) spectrometer at 298 K with tetramethylsilane (TMS) as the internal standard. Standard Bruker automated acquisition programs were used for all experiments. All one-dimensional spectra were composed by 64 K data points. The spectral width for ¹H was set at 6024 Hz and for ¹³C at 18519 Hz with acquisition times of 5.439 and 1.769 sec, respectively. The resolution of the spectra was 0.565 Hz per point for ¹³C and 0.184 Hz per point for ¹H. The numbers of scans for ¹H and ¹³C were 16 and 1024, respectively. The following abbreviations were used to explain the multiplicities in Fig. 2: s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet. Chemical shifts were assigned relative to TMS. Coupling constants (J) are reported in Hertz (Hz). The spectra and assignments were obtained with neat sample dissolved in different solutions (CDCl₃/DMSO-d₆/D₂O) with different concentrations.

Zhang X., Chingin K., Zhong D., Liang J., Ouyang Y, & Chen H. (2017). On the chemistry of 1-pyrroline in solution and in the gas phase. Scientific Reports, 7, 7675.

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Spectroscopic Analysis of Gamma-Irradiated Hydroxyapatite

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The ¹³C nuclear magnetic resonance (NMR) spectra of the γ-ray treated and untreated HA were recorded at 27 °C on a 500 MHz NMR spectrometer (DRX500 Avance, Bruker BioSpin GmbH, Rheinstetten, Germany). D2O (Sigma-Aldrich, St. Louis, MO, USA) was used as the solvent in all the NMR experiments. Fourier-transform infrared (FT-IR) spectra of the samples were detected using an infrared spectrophotometer (Spectrum one, Perkin Elmer, Waltham, MA, USA). Before tests, the γ-ray treated and untreated HA powders were mixed with KBr (Sigma-Aldrich, St. Louis, MI, USA) and compressed into disks. The wavelength range was set at 650–4000 cm⁻¹. Transmission mode spectra were obtained from 24 scans. To detect the UV-Vis absorption spectra of the γ-irradiated HA, samples were diluted in distilled water to a concentration of 0.2% (mg/mL). UV-Vis spectra were measured using a CT-2400 Spectrophotometer (Great Tide Instrument Co., Ltd., Taipei, Taiwan) at a wavelength range of 200 nm to 500 nm. During detection, distilled water was used as a reference.

Huang Y.C., Huang K.Y., Lew W.Z., Fan K.H., Chang W.J, & Huang H.M. (2019). Gamma-Irradiation-Prepared Low Molecular Weight Hyaluronic Acid Promotes Skin Wound Healing. Polymers, 11(7), 1214.

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Synthesis and Characterization of Catalysts

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All of the chemical materials used in this work were purchased from Merck and Fluka and used without further purification. Melting points were determined on an Electrothermal 9100 apparatus. IR spectra were obtained on an ABB FT-IR (FTLA 2000) spectrometer. ¹H NMR and ¹³C NMR spectra were recorded on a Bruker DRX-500 AVANCE at 500 and 125 MHz respectively, using TMS as internal standard and DMSO (D6) as solvent. Elemental analyses were carried out on Foss-Heraeus CHN–O-rapid analyzer instruments. The microscopic morphology of the catalyst was revealed using scanning electron microscope (SEM, Philips, XL-30) equipped with an energy dispersive X-ray detector (EDX). The transmission electron microscopy (TEM) image of the catalyst was obtained on a Philips EM208 transmission electron microscope under acceleration. Powder X-ray diffraction data were determined on a Rigaku D-max C III, X-ray Diffractometer using Cu Kα radiation (λ = 1.54 Å). Ultrasonication was performed using on a multiwave ultrasonic generator (Sonicator 3200; Bandelin, MS 73), equipped with a converter/transducer and titanium oscillator (horn), 12.5 mm in diameter, with an operation frequency of 20 KHz with a maximum power output of 200 W.

Abdolmohammadi S., Mirza B, & Vessally E. (2019). Immobilized TiO2 nanoparticles on carbon nanotubes: an efficient heterogeneous catalyst for the synthesis of chromeno[b]pyridine derivatives under ultrasonic irradiation. RSC Advances, 9(71), 41868-41876.

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Synthesis of Pyrazole Derivatives

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All chemicals were purchased from Merck (Germany) and were used without further purification. Melting points were measured on an Electrothermal 9100 apparatus. Elemental analyses for C, H and N were performed using a Heraeus CHN-O-Rapid analyzer. Mass spectra were recorded on an Agilent Technologies (HP) 5973 mass spectrometer operating at an ionization potential of 20 eV. IR spectra were recorded on a Shimadzu IR-460 spectrometer. ¹H and ¹³C NMR spectra were measured (in chloroform (CDCl₃) and dime-thyl sulfoxide (DMSO-d₆) solutions) with Bruker DRX-500 AVANCE (at 500.1 and 125.8 MHz) instruments. α-azidochalcones 1 as well as 3-amino-N-aryl-5-(phenylamino)-1H-pyrazole-4-carboxamides 2a–c and ethyl 3-amino-5-phenyl-1H-pyrazole-4-carboxylate 2d were obtained from synthetic methods reported in the literature^{81 (link)–83 (link)}.

Peytam F., Adib M., Shourgeshty R., Firoozpour L., Rahmanian-Jazi M., Jahani M., Moghimi S., Divsalar K., Faramarzi M.A., Mojtabavi S., Safari F., Mahdavi M, & Foroumadi A. (2020). An efficient and targeted synthetic approach towards new highly substituted 6-amino-pyrazolo[1,5-a]pyrimidines with α-glucosidase inhibitory activity. Scientific Reports, 10, 2595.

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Synthesis and Characterization of Compounds

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Two melting points were measured on an Electrothermal 9100 apparatus. IR spectra were recorded as KBr pellets on a Nicolet FTIR 100 spectrophotometer. ¹H NMR (500 MHz, 300 MHz) and ¹³C NMR (75 MHz) spectra were obtained using Bruker DRX-500 Avance and Bruker DRX-300 Avance spectrometers. All NMR spectra were recorded at r.t. in DMSO-d₆ and CDCl₃. Chemical shifts are reported in parts per million (δ) downfield from an internal TMS reference. Coupling constants (J values) are reported in hertz (Hz), and standard abbreviations were used to indicate spin multiplicities. Elemental analyses for C, H, and N were performed using a Heraeus CHN-O-Rapid analyzer. Mass spectra were recorded on a Finnigan-MATT 8430 mass spectrometer operating at an ionization potential of 70 eV. All chemicals and solvents were purchased from Merck or Aldrich and were used without further purification. Starting materials were synthesized according to the procedures reported in the literature.^{15–20 (link)} Single crystals of compounds 3d were formed in CH₂Cl₂.

Farajpour B, & Alizadeh A. (2022). Chemoselective and one-pot synthesis of novel coumarin-based cyclopenta[c]pyrans via base-mediated reaction of α,β-unsaturated coumarins and β-ketodinitriles. RSC Advances, 12(12), 7262-7267.

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Quantification of Primary Amine Groups in Dextran-Spermine

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H-NMR analysis and TNBS method were used to determine the formation of the primary amine groups. For TNBS method, dextran-spermine was dissolved in 0.1 M sodium bicarbonate solution (pH 8.5) to obtain solutions ranging 20–200 µg/mL. A standard calibration curve for l-lysine was plotted. 1% TNBS was added to 0.1 M sodium bicarbonate solution and 500 µg of the resulting solutions was added to 1 mL of each sample. The samples were incubated for 2 h at 37 °C. Finally, 500 µg sodium dodecyl sulfate (10% w/v) and 250 µL HCl (1 N) were added to each sample to stop the reaction. The absorbance of the samples was recorded at 345 nm using a spectrophotometer (UV–Vis-CARY50). The primary amine content was calculated according to the calibration curve. H-NMR spectra were recorded on a BRUKER DRX500 AVANCE (500 MHz) instrument using DDW as solvent. Values were recorded as ppm relative to internal standard (TMS).

Avazzadeh R., Vasheghani-Farahani E., Soleimani M., Amanpour S, & Sadeghi M. (2017). Synthesis and application of magnetite dextran-spermine nanoparticles in breast cancer hyperthermia. Progress in Biomaterials, 6, 75-84.

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Synthesis and Polymerization of Benzofulvenes

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The details of the preparation of benzofulvene derivatives and their spontaneous polymerization are described in (ESI†). NMR spectra were recorded with either a Bruker DRX-400 AVANCE or Bruker DRX-500 AVANCE spectrometer in the indicated solvents (TMS as internal standard): the values of the chemical shifts are expressed in ppm and the coupling constants (J) in Hz. An Agilent 1100 LC/MSD operating with an electrospray source was used in mass spectrometry experiments.

Fabrizi de Biani F., Reale A., Razzano V., Paolino M., Giuliani G., Donati A., Giorgi G., Mróz W., Piovani D., Botta C, & Cappelli A. (2018). Electrochemical and optoelectronic properties of terthiophene- and bithiophene-based polybenzofulvene derivatives. RSC Advances, 8(20), 10836-10847.

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NMR Spectra Analysis Protocol

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All reagents and solvents were purchased from Sigma-Aldrich and were used as received, with the exceptions noted. Merck TLC aluminum sheets, silica gel 60 F₂₅₄ were used for TLC. NMR spectra were recorded with either a Bruker DRX-400 Avance, a Bruker DRX-500 AVANCE, a Bruker AMX-600 Avance, or a Bruker Avance 900 spectrometer, working at 900.13 MHz frequency and equipped with a cryogenically cooled probe in the indicated solvents (TMS as internal standard).

Cappelli A., Paolino M., Reale A., Razzano V., Grisci G., Giuliani G., Donati A., Bonechi C., Lamponi S., Mendichi R., Battiato S., Samperi F., Makovec F., Licciardi M., Depau L, & Botta C. (2018). Hyaluronan-based graft copolymers bearing aggregation-induced emission fluorogens. RSC Advances, 8(11), 5864-5881.

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Characterization of Synthesized Compounds

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All chemicals used were of reagent grade. Yields refer to purified products and are not optimized. Melting points were determined in open capillaries on a Gallenkamp apparatus and are uncorrected. Merck silica gel 60 (230–400 mesh) was used for column chromatography. Merck TLC plates, silica gel 60 F₂₅₄ were used for TLC. NMR spectra were recorded by means of either a DRX 400 AVANCE or a Bruker DRX 500 AVANCE spectrometer in the indicated solvents (TMS as internal standard); the values of the chemical shifts (δ) are expressed in ppm and the coupling constants (J) in Hz. Mass spectra were recorded on an Agilent 1100 LC/MSD.

Razzano V., Paolino M., Reale A., Giuliani G., Donati A., Giorgi G., Artusi R., Caselli G., Visintin M., Makovec F., Battiato S., Samperi F., Villafiorita-Monteleone F., Botta C, & Cappelli A. (2018). Poly-histidine grafting leading to fishbone-like architectures. RSC Advances, 8(16), 8638-8656.

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