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Thermalert th 5 thermometer

Manufactured by Physitemp
Sourced in United States

The Thermalert TH-5 thermometer is a compact, handheld device designed for measuring temperature. It features a digital display and provides accurate temperature readings.

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4 protocols using thermalert th 5 thermometer

1

Analyzing Mouse Body Composition and Lipid Excretion

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Body composition was analysed in mice treated with Nnmt and control ASO for 5 weeks using an EchoMRI 3-in-1 instrument (Echo Medical Systems). To measure body composition after fasting, food was removed from Nnmt-ASO- or control-ASO-treated mice for 16h. Body temperature was measured intrarectally using a Thermalert TH-5 thermometer (Physitemp). To analyse faecal lipid excretion, lipid content of faeces was extracted using chloroform:methanol (2:1) and air-dried under a fume hood.
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2

Binge Dosing Regimen for METH Neurotoxicity

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A binge dosing regimen was used to induce METH neurotoxicity (Howard et al. 2011 (link); Howard et al. 2013a (link); Howard et al. 2013b (link)). This regimen reliably mimics aspects of the long-term neurotoxic effects of METH observed in humans, including loss of markers for DA and serotonin neurons and microglia activation (Marshall and O’Dell 2012 (link)). Briefly, animals were placed in plastic housing tubs (50 cm length × 40 cm width × 20 cm height; 4 rats/tub). Saline (0.9 %) or (±)-METH•HCl (7.5 mg/kg; dissolved in 0.9% saline; calculated as free base) was injected s.c. every two hours until a total of four injections were administered. Core body temperature was monitored rectally (Thermalert TH-5 thermometer, Physitemp, Clifton, NJ, USA) every hour during injections and for two additional hours after injections.
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3

AIT-Mediated Protection Against Anaphylaxis

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Three weeks after the last AIT session, mice were challenged by an i.p. injection of 10 IR cat dander allergen extract (or 52.4 μg birch pollen allergen extract) to measure AIT-mediated protection against anaphylaxis. Before the challenge, and at 20–30 min intervals thereafter, body temperature was measured rectally with a digital Thermalert TH-5 thermometer with a RET-3 probe (Physitemp, Huron, NJ). A time–temperature curve was plotted and the area under curve (AUC) was calculated in GraphPad Prism v7.02 from GraphPad Software (La Jolla, CA). One day after the challenge, mice were euthanized and spleens were harvested for restimulation of splenocytes and analysis of cytokine secretion.
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4

Measuring Mast Cell Activation and Temperature Changes in Mice

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For analysis of immediate mast cell activation, mice were gavaged once with the respective MPs preparations and sera taken 1 h after gavage were screened for mouse mast cell protease-1 (mMCP-1) using the mouse MCPT-1 Ready-set-go ELISA Kit (eBioscience, San Diego, CA) according to manufacturer's instructions. Rectal body temperatures were measured 30 min, 1 h, 3 h, 6 h, 8 h, 24 h, 48 h and 72 h after single gavage using a Thermalert TH-5 thermometer (Physitemp Instruments Inc., Clifton, NJ).
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