Sodium hydroxide, sodium borohydride 98%, chloramine T trihydrate, and 4-(dimethylamino) benzaldehyde were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mass spectrometry grade water, acetonitrile (MeCN), formic acid, methanol, acetic acid glacial, hydrochloric acid S.G 1.18 (~37%), and Sodium hydroxide were sourced from Fisher Scientific (Fair Lawn, NJ, USA). Perchloric acid 70% and propan-1-ol were purchased from BDH Chemicals Limited (Poole, England). Deionized water was obtained from a Milli-Q Ultra-pure water system (Dubuque, IA, USA). Dihydroxylysinonorleucine standard (DHLNL) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Pyridinoline (PYD) (95%) and deoxypyridinoline (DPD) (98%) standards were purchased from BOC Science (New York, NY, USA). Hydroxylysinonorleucine (HLNL), histidinohydroxylysinonorleucine (HHL), histidinohydroxymerodesmosine (HHMD) standards, were isolated and purified in the Protein Structure and Function Laboratory, Massey University’s School of Natural Sciences [16 (link)].
4 dimethylamino benzaldehyde
Manufactured by Merck Group
Sourced in United States, Germany
4-(dimethylamino) benzaldehyde is a chemical compound used in various laboratory applications. It is a crystalline solid with a yellow color. The compound is commonly used as a reagent in analytical and organic chemistry procedures.
Automatically generated - may contain errors
Lab products found in correlation
Chloramine t, by Merck Group (8 mentions)
Trans 4 hydroxy l proline, by Merck Group (7 mentions)
Chloramine t trihydrate, by Merck Group (6 mentions)
Glacial acetic acid, by Merck Group (4 mentions)
Antibiotic antimycotic solution, by Merck Group (4 mentions)
Hydrolysate, by Merck Group (4 mentions)
Ehrlich solution, by Merck Group (4 mentions)
Acetic acid, by Merck Group (4 mentions)
Trichloroacetic acid, by Merck Group (4 mentions)
Perchloric acid, by Merck Group (4 mentions)
1 propanol, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Merck Group (3 mentions)
L tryptophan, by Merck Group (3 mentions)
Benzaldehyde, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Nacalai Tesque (2 mentions)
Citric acid, by Merck Group (2 mentions)
Sodium acetate, by Merck Group (2 mentions)
Carbazole reagent, by Merck Group (2 mentions)
Borax sulphuric acid mixture, by Merck Group (2 mentions)
49 protocols using 4 dimethylamino benzaldehyde
1
Mass Spectrometric Analysis of Collagen Cross-links
2
Synthesis of Nanocatalyst Materials
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Titanium (IV) oxide (P25, Sinopharm Chemical Reagent Co., Ltd, analytically pure), zirconium nitrate pentahydrate (Aladdin, Z190748, 99.5%), cerium nitrate hexahydrate (Aladdin, C105378, 99.5%), sodium hydroxide (Macklin, S817977, ≥ 98%), salicylic acid (Macklin, S817529, 99.5%), potassium sodium tartrate tetrahydrate (Macklin, P816438, 99.5%), sodium nitroferricyanide(III) dehydrate (Macklin, S818341, 99.98% metals basis), sodium hypochlorite solution (Macklin, S828471, available chlorine 4.0%), ammonium chloride (Maclin, A801305, 99.8%), hydrazine monohydrate (Alfa Aesar, A14005, 98%), hydrochloric acid (Sinopharm Chemical Reagent Co., Ltd, 10011018, 36.0−38.0%), potassium hydroxid (Aladdin, P112287, 99.99% metals basis), sulfuric acid (Sinopharm Chemical Reagent Co., Ltd, 10021618, 95.0−98.0%), ethyl alcohol (Sinopharm Chemical Reagent Co., Ltd, 10009218, ≥ 99.7%), 4-(dimethylamino) benzaldehyde (Sigma-Aldrich, 156477, 99%), Nafion solution (Dupont), 211 Nafion membrane (Dupont), deionized (DI) water (Millipore, 18.2 MΩ cm), N2 gas (99.99%), Ar gas (99.99%). Ammonium sulfate ((NH4)2SO4, 99%), (15NH4)2SO4, 98 atom% 15N), and nitrogen-15N2 (98 atom% 15N) were purchased from Sigma-Aldrich. All chemical reagents were used as received without further purification.
3
Hydroxyproline Content Quantification
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The left lungs were removed and the sample was homogenized in 1 mL of PBS and hydrolyzed with 1 mL of HCl for 16 h at 120 °C. The supernatant was centrifuged at 10,000g for 5 min (Model 3740, KUBOTA, Tokyo, Japan), and 5 µL of the supernatant was aliquoted into a 96-well plate. After dispensing 5 µL hydroxyproline standard (Sigma-Aldrich) into each well of the 96-well plate, 5 µL citrate/acetate buffer (deionized distilled water supplemented with 238 mM Citric acid, Sigma-Aldrich, 1.2% glacial acetic acid, Sigma-Aldrich, 532 mM sodium acetate, Sigma-Aldrich, and 850 mM sodium hydroxide, Nacalai Tesque) and 100 µL chloramine T solution (1.0 mL deionized distilled water supplemented with 0.141 g chloramine T, Sigma-Aldrich, 1.0 mL 1-propanol, Sigma-Aldrich, and 8.0 mL citrate/acetate buffer) were added. After 30 min of incubation at 25 °C, 100 µL of Ehrlich's reagent (2.5 g 4-dimethylaminobenzaldehyde, Sigma-Aldrich, 9.3 mL 1-propanol, and 3.9 mL 70% perchloric acid, Sigma-Aldrich) was added and the mixture was incubated at 65 °C for 30 min. After 5 min at 25 °C, the absorbance was measured at 550 nm using a plate reader (iMARK, Bio-Rad, Hercules, CA, USA), as previously described [21 (link)].
4
Quantifying Interferon-Gamma-Induced Kynurenine
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CT26 cells or DCs were seeded in a 96-well plate at a cell density of 1 × 104 cells per well and exposed to 50 ng/mL recombinant mouse IFN-γ for 24 h. Subsequently, the old culture medium was replaced with a fresh medium that contained 200 nM NP-IDO-APT, NP-Scr-APT, or NPs. After 24 h, 100 μL supernatants were collected and mixed with 50 μL 30% (w/v) trichloroacetic acid, then the mixture was incubated at 65°C for 15 min. After centrifugated at 12000 rpm for 10 min, the supernatant was mixed with an equal volume of 2% (w/v) 4-(Dimethylamino)benzaldehyde (Sigma-Aldrich) in acetic acid. The reaction product was read on a plate reader at 490 nm. Kynurenine concentrations were determined from kynurenine standard curves.
5
Comprehensive Biochemical Assay Protocol
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Antimycotic antibiotic solution, decamplmaneh fetal bovine serum, Dulbecco's Modified Eagle Medium (DMED), carbazole reagent, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 4-(Dimethylamino)benzaldehyde, phosphate buffer solution (PBS), borax-sulphuric acid mixture, Ehrlich solution, hydrolysate, and dimethyl sulfoxide (DMSO), all of this was obtained from Sigma-Aldrich, USA.
6
Synthesis of Organic Compounds
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Chloramine T trihydrate, trans-4-Hydroxy-L-proline, 4-(dimethylamino) benzaldehyde and other standard chemicals were purchased from Sigma (Taufkirchen, Germany).
7
Vancomycin Assay and Purification
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Vancomycin (Assay 99.9%) pure powder was obtained as a gift from Egy pharma (Nasr City, Cairo, Egypt). Aconitic acid, 4-dimethylaminobenzaldehyde, phosphoric acid, acetic acid, boric acid, sodium hydroxide, cobalt chloride, and quinine sulfate were purchased from Sigma Aldrich (Steinheim, Germany). All chemicals and reagents were of analytical reagent grade and were used as received without further purification. Double distilled water was purified using a Milli-Q Plus Water Purification System (Millipore, Billerica, MA, USA).
8
Synthesis and Characterization of Benzaldehyde Derivatives
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Barbituric acid, 1,3-dimethylbarbituric acid and 4-(diethylamino)benzaldehyde were purchased from Acros Organics (Geel, Belgium). 4-(Dimethylamino)benzaldehyde and DMSO was purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). 4-(4-Morpholinyl)benzaldehyde, 4-(1-pyrrolidino)benzaldehyde and 4-(diethylamino)salicylaldehyde were purchased from Accela Chembio (Shanghai, China). Other solvents were purchased from Chem-supply (Adelaide, SA, Australia). All chemicals were used as received without further purification.
1H-NMR and 13C-NMR spectra were recorded by using a 400 MHz Bruker AV3HD-400 spectrometer (Billerica, MA, USA) at 298 K, with tetramethylsilane (TMS, δ = 0) as the internal standard. HRMS spectra were acquired by using a Thermo Scientific Q Exactive Plus Orbitrap LC–MS/MS instrument operating in ESI (Electrospray ionization) mode. UV-visible absorption spectra were recorded at room temperature on a Cary 300 UV-visible spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA) equipped with a 1.0 cm quartz cell. Fluorescence emission spectra were recorded on a Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA) and used 1.0 cm quartz cells. Data were plotted by using Origin 2019.
1H-NMR and 13C-NMR spectra were recorded by using a 400 MHz Bruker AV3HD-400 spectrometer (Billerica, MA, USA) at 298 K, with tetramethylsilane (TMS, δ = 0) as the internal standard. HRMS spectra were acquired by using a Thermo Scientific Q Exactive Plus Orbitrap LC–MS/MS instrument operating in ESI (Electrospray ionization) mode. UV-visible absorption spectra were recorded at room temperature on a Cary 300 UV-visible spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA) equipped with a 1.0 cm quartz cell. Fluorescence emission spectra were recorded on a Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA) and used 1.0 cm quartz cells. Data were plotted by using Origin 2019.
9
Chitosan-based Synthesis of Indole Derivatives
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Chitosan (MW. 100,000–300,000 Dalton) was obtained from Across Organics. (New Jersey, USA), Indole-3-carboxaldehyde, (purity 97%; M.W. 145.15) and 4-dimethylaminobenzaldehyde, (purity 99% M.W. 149.19) were acquired from Sigma Aldrich (Germany). Sodium hydroxide pellets (purity 99–100%), Sulfuric acid (98%), Ethanol (99.9%) were supplied from International co for Supp& Med. Industries, (Egypt).
10
Atractylenolide I Immunomodulatory Properties
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Atractylenolide I, (4aS,8aS)-3,8a-dimethyl-5-methylidene-4a,6,7,8-tetrahydro-4H-benzo[f][1] benzofuran-2-one (CAS No.73069-13-3, MF C15H18O2, m.w. (g/mol) 230.3022, HPLC 98 %) was purchased from Chengdu Best-Reagent Co. Ltd. (Chengdu, China); lipopolysaccharide (LPS), phytohemagglutinin (PHA), MTT (3-[4,5-dimethylthiazol-2-yl]- 2,5-diphenyl tetrazolium bromide), l -kynurenine and 4-dimethylaminobenzaldehyde were purchased from Sigma Chemical Co. (St. Louis, MO, USA); the rabbit polyclonal antibodies to NF-κB p65, phospho-NF-κB p65 (S536)(ab76302), Akt and phospho-Akt (S129) (ab133458), anti-GAPDH antibody [EPR6256] (ab128915) and anti-TLR4/MD-2 complex antibody[7E3](FITC)(ab105855) were purchased from Abcam plc.(Cambridge, MA, USA); Peroxidase-Conjugated AffiniPure Goat Anti-Rabbit IgG was purchased from ZSGB-BIO, Inc. (Beijing,China); ELISA kits for IL-6, IL-4, IL-10, IL-17A, TGF-β1 and VEGF were purchased from R&D Systems, Inc. (Minneapolis, MN 55413, USA); CD4-FITC/CD8-PE/CD3-PerCP (Catalog Number: 340298) for determining CD4/CD8/CD3 counts of T lymphocytes and FoxP3 Staining Kit—FoxP3-PE/CD4-FITC/CD25-APC (Catalog Number: 560133) for determining counts of Treg cells were purchased from Becton, Dickinson and Company (BD Biosciences, USA); CytoTox 96® Non-Radioactive Cytotoxicity Assay (G1781) was purchased from Promega Corporation (Madison, WI, USA).
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