Capsaicin procedures were conducted similar to previous reports and included application of a thick piece of non-porous dressing to the skin at one of two randomized dorsal non-dominant hand locations [22 (link)–27 (link)]. This dressing was a template for cream application and included a 6.25 cm2 hole cut into the center of it (used to standardize the area of application). Approximately 0.35 g of 10% capsaicin cream was applied inside this hole and evenly spread on the skin. The area was then covered by Tegaderm™ transparent dressing (3M Health Care, St. Paul, MN, USA). Pain induced by topical capsaicin varies strongly as a function of skin temperature, thus a peltier-device heating element (Medoc US, Minneapolis, MN, USA) was strapped onto the area [28 (link)]. This device was held at a constant temperature of 40°C during the session. Following completion of each session, the capsaicin cream was removed from the skin.
Tegaderm transparent dressing
Manufactured by 3M
Sourced in United States
Tegaderm™ is a transparent dressing manufactured by 3M. It provides a protective barrier to help prevent contamination of wounds and support the healing process.
Automatically generated - may contain errors
Lab products found in correlation
Somnopentyl, by Kyoritsu Seiyaku (3 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Thermochron ibutton ds1992l, by Analog Devices (1 mentions)
Matrigel, by Corning (1 mentions)
Activpal, by PAL Technologies (1 mentions)
Activpal3, by PAL Technologies (1 mentions)
Palstickies, by PAL Technologies (1 mentions)
13 protocols using tegaderm transparent dressing
1
Topical Capsaicin Procedure for Pain Induction
2
Chitosan-based Hydrogel Wound Healing
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A total of fifteen healthy Sprague–Dawley rats (7–8 weeks old) were included in the approved in vivo study, authorized by the Animal Ethics Committee of Southern Medical University under approval/accreditation number SCXK(YUE)2021−0041. To assess the effect of the LC−MSN−incorporated hydrogel on wound healing, three subgroups were established: the control, CS hydrogel, and CS/LC−MSN hydrogel groups. The rats were anesthetized by intraperitoneal injection of pentobarbital sodium (3% w/v, 40 mg/mL). Following shaving, four full−thickness skin wounds (round, diameter = 6 mm) were created symmetrically on the dorsal skin using a biopsy punch. The hydrogels were then applied to the wounds, while the control wounds remained untreated but were covered with Tegaderm transparent dressing (3M Health Care, Germany) to prevent infection. The healing progress of each wound was observed and photographed at 0, 3, 7, and 14 days. The degree of wound closure was quantitatively determined by measuring the wound area using ImageJ software and calculated using the following equation:
where A0 represents the initial wound area and At is the wound area at the specific time points (0, 3, 7, and 14 days). At the end of the experiment, the rats were euthanized using an isoflurane overdose. The wound sites were excised for subsequent histological and immunohistochemistry analyses.
where A0 represents the initial wound area and At is the wound area at the specific time points (0, 3, 7, and 14 days). At the end of the experiment, the rats were euthanized using an isoflurane overdose. The wound sites were excised for subsequent histological and immunohistochemistry analyses.
3
Dorsal Wound Healing in Diabetic Animals
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Following 3–6 weeks of diabetic induction, animals underwent Tufts University IACUC-approved dorsal wounding surgeries: after anesthesia induction with a telazol/ketamine/xylazine cocktail, dorsal hair was removed with depilatory cream, followed by shaving. Under general anesthesia with isoflurane, the paraspinal area was prepared and draped sterilely, and full-thickness (5.0 mm deep) excisional wounds were created in the animals’ backs using sterile, stainless steel biopsy punches (8.0 mm-diameter) or a sterile No. 11 surgical blade (2.0 cm × 2.0 cm square wounds). Following hemostasis by manual compression, wounds were covered using 10 % compound tincture of benzoin adhesive and Tegaderm transparent dressing (3 M, St. Paul, MN) and animals were placed in protective jackets. Comb1 (1.0 mg/mL) and UN3 (284 μg/mL) peptides (synthesized by Anaspec, Fremont, CA) were suspended in autoclaved 4 % carboxymethylcelluose and administered to wounds daily by injection beneath the Tegaderm bandage, for a period of 3–7 days. Sterile saline served as a negative control.
4
Induction of Allergic Skin Inflammation in Mice
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A mouse AD model was generated as shown in Figure 1A , which was modified from previous reports (43 (link)–45 (link)). The hair on the back skin of anesthetized mice was clipped by using electric clippers. Residual hair was depilated by using a Nair hair remOVAl cream. Antigen challenge was performed by using one square centimeter of gauze containing 100 μg of CRE (Greer Laboratories) or OVA (MilliporeSigma) in PBS pipetted onto a 1 cm square sterile gauze pad and placed on the dorsal shaved skin. Control mice received PBS alone. The patched skin area was sealed with a Tegaderm Transparent dressing (3M HealthCare) using bandages. These procedures were repeated twice a week for 3 weeks with 1-week interval. The severity of skin inflammation (e.g., erythema/hemorrhage, eruption, scarring/dryness) was evaluated using EASI score on day 2 after the patch remOVAl as 0 (no symptom), 1 (mild), 2 (intermediate), or 3 (severe). The total score of skin lesions was designated as the sum of individual scores. After final evaluation, mice were sacrificed, and serum, lesion, and nonlesion skin tissues were collected.
5
Monitoring Skin Temperature Changes
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In this study, proximal and distal skin temperatures were defined as the thermal measurements obtained from the abdominal surface area and distal region of the ventral forearm, respectively. Proximal and distal skin temperatures were measured at 1-min and 3-min intervals, respectively, using wireless temperature loggers (Thermochron iButton DS1992L; Maxim Integrated, CA, US). The accuracy, range, and resolution of the temperature logger were ±0.5 °C, −10 to +65 °C, and 0.0624 °C, respectively. The temperature logger was attached to the right upper quadrant of the abdominal surface using a Tegaderm transparent dressing (3M, St. Paul, MN, US). The logger for distal skin temperature was attached to the actigraph wristband using a plastic attachment, ensuring that the temperature logger sensor touched the ventral skin surface. The means of the proximal and distal skin temperatures during the daytime and 1–60 min before and after bathing were calculated based on the self-reported diary.
6
Burn Wound Infection Model in Rats
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Adult male Wistar rats (500–650 g) were anesthetized by intraperitoneal injection of Ketamine plus Rompun. The dorsum of the rat was shaved with hair clippers. For each rat, four burned wounds were created by pressing 1×1 cm2 copper plates preheated to 120°C on its back for 12 seconds. The wound area was protected using a polyethylene foam sheet with a 1×1 cm2 hole in the middle to create a well for infection. 20 µl of late log bacterial suspension (108 cells/ml) was applied evenly on each wound well and each well was covered with Tegaderm™ transparent dressing (3M™, USA) to prevent contamination by other microbes and to maintain humidity. After overnight infection, the wound was washed with PBS and superficial burned skin was removed, weighed, and homogenized in PBS. Aliquots of homogenates were spread on LSW−, and CFU was determined after overnight incubation at 37°C.
7
Wound Healing with Stem Cells
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Female BALB/c nu/nu mice (4‐week‐old) were anaesthetized with sodium pentobarbital (40 mg/kg) via intraperitoneal injection, and skin wounds (symmetrical, full‐thickness, 5‐mm diameter) were created on the disinfected back using a sterile and disposable 5‐mm biopsy punch (cat no. 50.005; Gyneas). Treated or untreated SKPs (2 × 106) and newborn‐mouse‐derived Epi‐SCs (1 × 106) were encapsulated in 30 μL of thawed Matrigel (Corning Inc), and the cell‐Matrigel mixture was incubated at 37°C for 30 minutes prior to implantation into the full‐thickness excisional wound. The wound was then covered with a tegaderm transparent dressing (2‐3/8 × 2‐3/4 inches; 3 M, St. Paul, MN, USA) and a self‐adhering elastic bandage (2.20 yards; Johnson & Johnson). After 3 weeks, the newborn hair was photographed under a stereoscopic microscope (SZM7045TR; Keyence), and the mice from whom hair samples were obtained for photographing, fixing and paraffin‐embedding were sacrificed.
8
Accelerometer-based Physical Activity Assessment
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Physical activity was assessed with the ActivPal™ (PAL Technologies Ltd, Glasgow, UK), a small, lightweight (35 × 53 × 7 mm, 15 g) piezoelectric triaxial accelerometer. The monitor was attached to participants’ upper thigh with hydrogel adhesion pads (PALstickies™, PAL Technologies Ltd, Galsgow, UK) and a Tegaderm™ transparent dressing (3M, St Paul, Minneapolis, USA). Accelerometry measurements were made at a sampling rate of 15 Hz and summarised into 15 s epochs, then downloaded and analysed using the manufacture’s software (ActivPal3™ version 7.1.18, PAL Technologies, Glasgow, UK).
9
Murine Wound Healing Model
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Mice were anesthetized by intraperitoneal injection of 40 mg/kg sodium pentobarbital (Somnopentyl, Kyoritsu Seiyaku Corporation, Tokyo, Japan), and sustained by inhalation anesthesia of isoflurane (Isoflurane, Mairan Pharma, Osaka, Japan). The dorsal hair was shaved to fully expose the skin, which was then rinsed with 70% ethanol. Four full-thickness wounds extending to the panniculus carnosus were created on each mouse using a 6-mm-diameter biopsy punch (Biopsy Punch, Kai Industries Co., Ltd., Gifu, Japan) under sterile conditions and covered with a polyurethane film (Tegaderm Transparent Dressing, 3 M Health Care, St. Paul, MN, USA) and an elastic adhesive bandage (Hilate, Iwatsuki, Tokyo, Japan) for an occlusive dressing. At various time points, mice were sacrificed by overdose of isoflurane, and the wound tissue was collected by excising a 1-cm-square section of skin using scissors and a surgical knife. The tissue was then processed for histopathological analysis and measurement of cytokine concentrations.
10
Excisional Wound Healing in Mice
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Mice were anesthetized with an intraperitoneal injection of 40 mg/kg sodium pentobarbital (Somnopentyl, Kyoritsu Seiyaku Corporation, Tokyo, Japan), and sustained by inhalation anesthesia of isoflurane (Isoflurane, Mairan Pharma, Osaka, Japan). The dorsal hair was shaved to fully expose the skin, which was then rinsed with 70% ethanol. Four full-thickness wounds extending to the panniculus carnosus were created using a 6-mm-diameter biopsy punch (Biopsy Punch, Kai Industries Co., Ltd., Gifu, Japan) under sterile conditions. The injured areas were covered with a polyurethane film (Tegaderm Transparent Dressing, 3M Health Care, Saint Paul, MN, USA) and an elastic adhesive bandage (Hilate, Iwatsuki, Tokyo, Japan) for an occlusive dressing. At various time points, mice were sacrificed and the wound tissue was collected by excising a 1-cm-square section of skin using scissors and a surgical knife. The tissue was then processed for histopathological analysis and measurement of cytokine concentrations.
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