Aprotinin

Manufactured by ITW Reagents

Aprotinin is a protease inhibitor commonly used in laboratory settings. It functions by inhibiting the activity of various proteolytic enzymes, which can be useful in various experimental and analytical procedures.

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Lab products found in correlation

H d val leu lys p nitroanilide s 2251, by Innovative Research (2 mentions) Sch 79797, by Cayman Chemical (2 mentions) Lipoteichoic acid lta from s aureus, by InvivoGen (1 mentions) Odn1826, by InvivoGen (1 mentions) Lps from e coli serotype 055 b5, by Merck Group (1 mentions) Rwj 56110, by R&D Systems (1 mentions) Plasmin, by Innovative Research (1 mentions) Human ei tpa, by Innovative Research (1 mentions) Dizocilpine mk 801, by Cayman Chemical (1 mentions)

2 protocols using aprotinin

Purification and Characterization of Plasminogen and tPA

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Glu-Plasminogen was purified from human plasma as previously described [27 (link)]. Human tPA, which is produced in CHO cells and 95% in the two-chain form, was from Molecular Innovations. Lipopolysaccharide (LPS) serotype 055:B5 from E. coli was from Sigma-Aldrich. The TLR2 ligand, lipoteichoic acid (LTA) from S. aureus and the TLR9 ligand, ODN 1826, were from InvivoGen. Amiloride (AMD) was from Sigma-Aldrich. Mouse uPAR-specific antibody (cat. AF534) and control IgG (cat. AB105C) were from R&D Systems. α-Enolase-specific polyclonal antibody was from Invitrogen (cat. 3810T). Rabbit polyclonal antibody that targets the C-terminus of actin was from Sigma-Aldrich (cat. A2066). ε-Aminocaproic acid (εACA) was from MP Biomedicals. Aprotinin was from PanReac AppliChem. SCH 79797 was from Cayman Chemicals. The plasmin-specific chromogenic substrate, H-D-Val-Leu-Lys-p-nitroanilide (S-2251) and mouse uPA ATF were from Molecular Innovations.

Pontecorvi P., Banki M.A., Zampieri C., Zalfa C., Azmoon P., Kounnas M.Z., Marchese C., Gonias S.L, & Mantuano E. (2019). Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokines. Journal of Neuroinflammation, 16, 257.

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Plasminogen Activation Biochemical Assay

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Enzymatically-active human tPA, which is produced in CHO cells and 95% in the two-chain form, and human EI-tPA, which carries the S478→A mutation and is 90% in the single-chain form, were from Molecular Innovations. Glu-Plasminogen was purified from human plasma as previously described.^{28 (link)} The purified Plasminogen preparations studied here were unresolved mixtures of the two major glycoforms. Plasmin (>10 International units/ mL), which was pre-activated with immobilized low molecular weight urokinase, was from Molecular Innovations. LPS serotype 055:B5 from E. coli was from Sigma-Aldrich. Dizocilpine (MK-801) was from Cayman Chemical. ε-aminocaproic acid (ƐACA) was from MP Biomedicals. Aprotinin was from PanReac AppliChem. SCH 79797 was from Cayman Chemicals and RWJ 56110 from R&D Systems. The PAR1 agonist peptide, TFLLR, and the control peptide, RLLFT, were from R&D Systems. The Plasmin-specific substrate, H-D-Val-Leu-Lys p-nitroanilide (S-2251), was from Molecular Innovations.

Zalfa C., Azmoon P., Mantuano E, & Gonias S.L. (2019). Tissue-type plasminogen activator neutralizes LPS but not protease-activated receptor-mediated inflammatory responses to plasmin. Journal of leukocyte biology, 105(4), 729-740.

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