Adipic dihydrazide

Manufactured by Merck Group

Sourced in United States

Adipic dihydrazide is a chemical compound used as a laboratory reagent. It functions as a reducing agent and can be used in various analytical and synthetic applications. The detailed technical specifications and intended uses of this product are not available without further research.

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Lab products found in correlation

5 protocols using adipic dihydrazide

Gelatin-Based Hydrogel Synthesis

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Gelatin A from porcine
skin, GG (Gelzan CM Gelrite, M_w 1000 g
mol^–1), spermidine trihydrochloride (SPD), sucrose,
adipic dihydrazide (ADH), carbodihydrazide (CDH), dimethyl sulfoxide
(DMSO), ethylene glycol, 1-ethyl-3-[3-(dimethylamino)-propyl]-carbodiimide
(EDC), hydroxylamine hydrochloride, N-hydroxybentzotriazole
(HOBt), 4-hydroxybenzaldehyde, deuterium oxide (99.9 atom % D, contains
0.05 wt % 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid, sodium salt),
hydrochloric acid (HCl), sodium hydroxide (NaOH), sodium chloride
(NaCl), and sodium periodate (NaIO₄) were purchased from
Sigma-Aldrich (St. Louis, MO, USA). Dialysis membrane (Spectra/Por
12–14 kDa) was purchased from Spectrum Laboratories (Rancho
Dominguez, CA, USA).

Koivisto J.T., Gering C., Karvinen J., Maria Cherian R., Belay B., Hyttinen J., Aalto-Setälä K., Kellomäki M, & Parraga J. (2019). Mechanically Biomimetic Gelatin–Gellan Gum Hydrogels for 3D Culture of Beating Human Cardiomyocytes. ACS Applied Materials & Interfaces, 11(23), 20589-20602.

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Hyaluronic Acid-Doxorubicin Conjugate Synthesis

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Hyaluronic acid (HA, MW = 100 kDa) was purchased from Lifecore Biomedical, LLC (Chaska, MN, USA). Doxorubicin (DOX) hydrochloride was obtained from Chemieliva Pharmaceutical Co., Ltd. (Chongqing, China). Tris(4-(dimethylamino) phenyl) methylium chloride (crystal violet), ethylenediaminetetraacetic acid (EDTA), paraformaldehyde (PFA), 4,6-diamidino-2-phenylindole (DAPI), and Alamar blue were purchased from Thermo Fisher Scientific (Waltham, CA, USA). Hydrazine, adipic dihydrazide, 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM Cl), bovine serum albumin (BSA), methyl cellulose (4000 cP), and Triton 100-X were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture media and reagents were obtained from Gibco (ThermoFisher Scientific, Waltham, CA, USA). Anti-LAMP-1 (lysosomal-associated membrane protein 1) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany), and FITC anti-mouse/human CD44 antibody was purchased from Biolegend (San Diego, CA, USA). If not specified, all the reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Malfanti A., Catania G., Degros Q., Wang M., Bausart M, & Préat V. (2022). Design of Bio-Responsive Hyaluronic Acid–Doxorubicin Conjugates for the Local Treatment of Glioblastoma. Pharmaceutics, 14(1), 124.

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Hyaluronic Acid-based Nanoparticles for Doxorubicin Delivery

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Hyaluronic acid, propylene sulfide, adipic
dihydrazide, caprolactone, N-Boc-ethanolamine, doxorubicin
hydrochloride (DOX·HCl), dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine
(DMAP), ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride
(EDC·HCl), and N-hydroxysuccinimide (NHS) were
purchased from Sigma-Aldrich Co., Ltd. (Beijing, China). N,N-Dimethylformamide (DMF) and triethylamine (TEA)
were purchased from Alfa Aesar Co., Ltd. (Shanghai, China). 2,7-Dichlorofluorescin
diacetate (DCFH-DA) was purchased from Yeasen Biotechnology Co., Ltd.
(Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI), propidium
iodide (PI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT), and TUNEL agents were obtained from Solarbio Co., Ltd.
(Beijing, China). Cell culture agents were purchased from GIBCO. Antibodies
were acquired from Abcam Co., Ltd. (Shanghai, China).

Yu Y., Feng Z., Liu J., Hou X., Zhou X., Gao J., Wang W., Zhang Y., Li G, & Liu J. (2021). γ-Ray-Triggered Drug Release of Reactive Oxygen Species-Sensitive Nanomedicine for Enhanced Concurrent Chemoradiation Therapy. ACS Omega, 6(30), 19445-19457.

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Modular Hydrogel Tissue Engineering

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Gelatin A from porcine skin, gellan gum (Gelzan CM Gelrite), adipic dihydrazide (ADH), dimethyl sulfoxide (DMSO), ethylene glycol, spermidine trihydrochloride (SPD), 1-ethyl-3-[3 dimethylamino)-propyl]–carbodiimide(EDC), hydroxylamine hydrochloride, N-hydroxybentzotriazole (HOBt), hydrochloric acid, sodium hydroxide, sodium chloride (NaCl), and sodium periodate were obtained from Sigma Aldrich. Geltrex was obtained from Thermo Fisher.

Belay B., Koivisto J.T., Parraga J., Koskela O., Montonen T., Kellomäki M., Figueiras E, & Hyttinen J. (2021). Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels. Scientific Reports, 11, 6538.

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Hydrogel Preparation with Functionalized PEO

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Poly(ethylene oxide) (PEO) polymer with hexa-thiols (MW = 10 kDa) was purchased from Sunbio Inc. (Seoul, Korea). While the chemicals of carboxymethyl cellulose sodium salt (CMC) (MW = 90 kDa), adipic dihydrazide (ADH) (MW = 174 kDa), acrylic acid (MW = 72 Da), adipic acid dihydrazide (ADH), dimethyl sulfoxide (DMSO) and esterase solution from porcine liver (177 unit/mg) were purchased from Sigma-Aldrich Chemical Co. (MO, USA), N-(3-diethylpropyl)-N-ethylcarbodiimide hydrochloride (EDC) and 1-hydroxybenzotriazole hydrate (HOBt) were obtained from Fluka Chemie GmbH (Buchs, Switzerland). While DMEM-604 and penicillin-streptomycin were purchased from Lonza Korea (Switzerland), cell counting kit-8 (CCK-8) solution and live & dead viability/cytotoxicity kit for mammalian cells were bought from Dojindo Laboratories (Japan) and Invitrogen (USA), respectively. Fetal bovine serum (FBS) and in vitro toxicology assay kits such as bromodeoxyuridine (BrdU), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Neutral red were purchased from Sigma-Aldrich (MO, USA), Roche (Germany) and Gibco BRL (Australia), respectively. All chemicals were employed as received.

Lee S.Y., Bang S., Kim S., Jo S.Y., Kim B.C., Hwang Y, & Noh I. (2015). Synthesis and in vitro characterizations of porous carboxymethyl cellulose-poly(ethylene oxide) hydrogel film. Biomaterials Research, 19, 12.

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