Crystal violet methanol solution

Manufactured by Merck Group

Crystal violet-methanol solution is a laboratory reagent used in various staining and analytical procedures. It is a purple-colored solution composed of the dye crystal violet dissolved in methanol. This solution is commonly used in microbiology and cell biology applications, such as Gram staining for the identification of bacteria.

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Lab products found in correlation

Paraformaldehyde (pfa), by Santa Cruz Biotechnology (1 mentions) Lapatinib, by Selleck Chemicals (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Spark m10, by Tecan (1 mentions) Azd0156, by MedChemExpress (1 mentions)

3 protocols using crystal violet methanol solution

Cell Viability and Proliferation Assays

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CRC XLs were seeded at different densities (5-7 × 10³ cells per well) in 100 μl complete growth medium in 96-well plastic culture plates at day 0. The following day, serial dilutions of the indicated drugs were added to the cells in serum-free medium while medium-only was included as controls. Plates were incubated at 37 °C in 5% CO₂ for 5 or 6 days, after which cell viability was assessed by measuring ATP content through CellTiter-Glo Luminescent Cell Viability assay (Promega). Luminescence was measured by SPARK M10 (Tecan) plate reader. Treated wells were normalized to untreated/DMSO treated wells.
For long-term proliferation assays, cells were seeded in 24-wells plates (1-2.5 × 10⁴ cells per well) and cultured in the absence and presence of drugs as indicated. Wells were fixed with 4% paraformaldehyde (Santa Cruz) and stained with 1% crystal violet-methanol solution (Sigma-Aldrich) after 2 weeks. All assays were performed independently at least three times.
Cetuximab and trastuzumab were obtained from the Pharmacy at Niguarda Cancer Center in Milan, Italy. Lapatinib was purchased from Selleck Chemicals.

Lazzari L., Corti G., Picco G., Isella C., Montone M., Arcella P., Durinikova E., Zanella E.R., Novara L., Barbosa F., Cassingena A., Cancelliere C., Medico E., Sartore-Bianchi A., Siena S., Garnett M.J., Bertotti A., Trusolino L., Di Nicolantonio F., Linnebacher M., Bardelli A, & Arena S. (2019). Patient-derived xenografts and matched cell lines identify pharmacogenomic vulnerabilities in colorectal cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(20), 6243-6259.

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Cetuximab Cytotoxicity Assay in LIM1215 Cells

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LIM1215 cells were seeded (4 × 10³ per well in 24-well culture plates). After 24 h, a serial dilution of cetuximab was added to the cells, and medium-only in control wells. Four technical replicates were plated per experiment. Plates were incubated at 37 °C in 5% CO₂ for 8 days, after which cells were fixed with 4% paraformaldehyde and stained with 1% crystal violet-methanol solution (Sigma-Aldrich).

Van Emburgh B.O., Arena S., Siravegna G., Lazzari L., Crisafulli G., Corti G., Mussolin B., Baldi F., Buscarino M., Bartolini A., Valtorta E., Vidal J., Bellosillo B., Germano G., Pietrantonio F., Ponzetti A., Albanell J., Siena S., Sartore-Bianchi A., Di Nicolantonio F., Montagut C, & Bardelli A. (2016). Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer. Nature Communications, 7, 13665.

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Colibactin Sensitivity and Drug Proliferation Assays

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On day 0, cells were plated at different densities in 24-multiwell plates. For colibactin sensitivity assay, the following day cells were infected with colibactin-producing or empty-vector bacteria at indicated MOIs as described above. Following 4 h of infection, residual bacteria were removed and cells were incubated at 37°C 5% CO₂ for 7 days in their respective complete medium supplemented with 20μg/mL gentamicin. For drug proliferation assays, serial dilutions of each drug were added to the cells, which were then incubated at 37°C in 5% CO₂ for 7 days. Following incubation, cells were fixed with 4% paraformaldehyde and stained with 1% Crystal Violet-Methanol solution (Sigma-Aldrich). Crystal Violet was then solubilized with 10% acetic acid and absorbance was quantified at 595nm. Oxaliplatin (S1224), SN-38 (S4908), olaparib (AZD2281, Ku-0059436, S1060), and ATM inhibitor AZD0156 were purchased from MedChemExpress. The sensitivity profile to chemotherapeutic agents shown in Figure 6A was derived from ref. ³³ (link).

Sogari A., Rovera E., Grasso G., Mariella E., Reilly N.M., Lamba S., Mauri G., Durinikova E., Vitiello P.P., Lorenzato A., Avolio M., Piumatti E., Bonoldi E., Aquilano M.C., Arena S., Sartore-Bianchi A., Siena S., Trusolino L., Donalisio M., Russo M., Di Nicolantonio F., Lembo D, & Bardelli A. (2024). Tolerance to colibactin correlates with homologous recombination proficiency and resistance to irinotecan in colorectal cancer cells. Cell Reports Medicine, 5(2), 101376.

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