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Flagellin

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Flagellin is a protein subunit that forms the main structural component of bacterial flagella. It plays a key role in bacterial motility and chemotaxis.

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Lab products found in correlation

Nigericin, by Thermo Fisher Scientific (1 mentions) Imiquimod, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Mcc950, by Thermo Fisher Scientific (1 mentions) Mouse il 1β elisa kit, by Thermo Fisher Scientific (1 mentions) Ag 25b 0006 c100, by Adipogen (1 mentions) Ag 20b 0014 c100, by Adipogen (1 mentions) Anti flag antibody f1804, by Merck Group (1 mentions) Human il 1β elisa kit, by R&D Systems (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Ag 20b 0042 c100, by Adipogen (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Malp 2, by Enzo Life Sciences (1 mentions) Cpg odn 1826, by TIB Molbiol (1 mentions) High purity flagellin, by Enzo Life Sciences (1 mentions)

4 protocols using flagellin

1

Inflammasome Activation Pathway Protocol

Cited 1 time
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ODZ was provided by Professor Sang-Kyu Ye (Department of Pharmacology, Seoul National University College of Medicine, Seoul, Republic of Korea) [21 (link)]. Penicillin-streptomycin, trypsin-EDTA, Fetal bovine serum, RPMI 1640, DMEM-high glucose pyruvate, and Opti-MEM were purchased from Gibco (Grand Island, NY, USA). LPS (O111:B4), ATP and DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nigericin, nano-SiO2, imiquimod, poly(dA:dT), MSU crystal, Y-VAD-CMK, MCC950, lipofectamine-2000, and mouse IL-1β ELISA kit were purchased from InvitroGen (San Diego, CA, USA). Flagellin was purchased from Adipogen (San Diego, CA, USA). Mouse IL-1β antibody (AF-401-NA) and human IL-1β ELISA kit were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against mouse caspase-1 (AG-20B-0042-C100), ASC (AG-25B-0006-C100), and NLRP3 (AG-20b-0014-C100) were purchased from Adipogen (San Diego, CA, USA), and mGSDMD (ab209845) was purchased from Abcam (Cambridge, UK). Anti-FLAG antibody (F1804) and β-Actin antibody (SC-4778) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Santa Cruz biotechnology (Dallas, TX, USA), respectively.
Kang J.H., Lee S.B., Seok J., Kim D.H., Ma G., Park J., Jeong A.J., Ye S.K, & Kang T.B. (2023). Novel Activity of ODZ10117, a STAT3 Inhibitor, for Regulation of NLRP3 Inflammasome Activation. International Journal of Molecular Sciences, 24(7), 6079.
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2

Flagellin and MALP-2 Immunoassay Protocol

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High purity flagellin (isolated from Salmonella typhimurium strain 14028) and MALP-2 were purchased from Enzo Life Sciences (USA). For use in ELISA of anti-flagellin antibodies, flagellin was purchased from AdipoGen Life Sciences (Switzerland). LPS from Escherichia coli (026:B6), bafilomycin A1, depleted zymosan, and ovalbumin were purchased from Sigma-Aldrich (USA). CpG-ODN (1826) was from TIB Molbiol (Germany). Biotinylated mouse anti-TLR5 monoclonal antibody (clone ACT5) was a gift from Kensuke Miyake (University of Tokyo, Japan). For flow cytometry, following fluorophore-conjugated antibodies were used: Ly6C-PB (clone HK1.4), Ly6G-APC (clone RB6-8C5) from eBioscience (USA); CD11b-PECy7 (clone M1/70), CD11c-PE (clone HL3) from BD Biosciences (USA); B220-PB (clone RA3-6B2) from TONBO Biosciences (USA); mPDCA1-APC (clone JF05-1C2.4.1) from Miltenyi Biotec (Germany). Following antibodies were used for immunoblotting: IRF3 (clone FL-425) from Santa Cruz Biotechnology (USA); IRF7 (clone EPR4718) from Abcam (UK); phospho-IRF3 (clone 4D4G), phospho-IRF7 (polyclonal) from Cell Signaling Technology (USA). Anti-mouse CD16/32 antibody was purchased from BioLegend (USA). Accudenz was from Accurate Chemical and Scientific Corporation (USA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse Ig antibodies were purchased from Southern Biotech (USA).
Kang W., Park A., Huh J.W., You G., Jung D.J., Song M., Lee H.K, & Kim Y.M. (2020). Flagellin-Stimulated Production of Interferon-β Promotes Anti-Flagellin IgG2c and IgA Responses. Molecules and Cells, 43(3), 251-263.
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3

Immunoblotting of NLRC4 and Related Proteins

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Anti-NLRC4 Ab, anti–phospho-NLRC4 Ab, anti-LGR5 Ab, V5 Tag mAb, and DYKDDDDK Tag mAb were from Thermo Fisher Scientific. Anti-PKCδ Ab (Proteintech), anti–IL-18 Ab (Abcam), anti-cleaved caspase-1 Ab, and anti-cleaved caspase-8 Ab (Cell Signaling Technology), and β-actin Ab (Santa Cruz Biotechnology) were purchased. HiPerFect transfection reagent (Qiagen), flagellin (AdipoGen Life Sciences), Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific), TRIzol (Life Technologies), Percoll (Solarbio Life Sciences), gentle cell dissociation and IntestiCult organoid growth medium (STEMCELL Technologies), a pcDNA3.1/V5-His TOPO TA expression kit (Invitrogen), Pierce magnetic RNA-protein pull-down kits (Invitrogen and Thermo Fisher Scientific), and a DIG Northern starter kit (Roche) were purchased. Oligonucleotides are listed in Supplemental Table I.
Wang Y., Gao Y., Su X., Hao Y., Zhang Y, & Yang R. (2024). LNCGM1082 in Gut Epithelial Cells Promotes Expulsion of Infected Epithelial Cells and Release of IL-18. ImmunoHorizons, 8(1), 35-46.
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4

High-throughput screening of natural products against TLR4 signaling

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PrimeStar GXL polymerase, Great EscAPe SEAP Chemiluminescent kit and In-Fusion HD cloning kit reagent were obtained from TaKaRa Bio, Japan. pEB-Multi-Neo and G418 were obtained from Wako, Japan. Normocin, Blasticidine, Hygromycin B Gold, QUANTI-Blue, HEK293T-TLR4-MD2-CD14, pNiFty2-SEAP and pNiFty2-56K-SEAP were obtained from InvivoGen, San Diego, CA, USA. LPS were obtained from Sigma-Aldrich, St. Louis, MO, USA. Recombinant human IFN-beta was obtained from Peprotech, Rocky Hill, NJ, USA. Recombinant human CIRP and Flagellin (high purity) were obtained from Adipogen life sciences, San Diego, CA, USA. Mouse S100A13 and PTMA were generated from E coli and then, were subjected to endotoxin removal resin. In both of them the endotoxin concentration was below 1EU per microgram protein. Natural products library was kindly gifted from Dr. Yamada and Prof. Takeda.
Kurata R., Kumagai A., Cui X., Harada M., Nagai J., Yoshida Y., Ozaki K.I., Tanaka Y, & Yonezawa T. (2018). Establishment of Novel Reporter Cells Stably Maintaining Transcription Factor-driven Human Secreted Alkaline Phosphatase Expression. Current pharmaceutical biotechnology, 19(3).
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