The methods for OKR recording to assess visual function were similar to prior studies (7 (link)). OKR experiments were performed using OptoMotry software and apparatus (Cerebral Mechanics Inc., Medicine Hat, AB, Canada). OKR function was determined by the highest spatial frequency at which mice track a 100% contrast grating projected at varying spatial frequencies, and data are reported as cycles/degree.
Optomotry software and apparatus
Manufactured by Cerebral Mechanics
Sourced in Canada
The OptoMotry software and apparatus is a tool for measuring visual function in animals. It is designed to quantify the optokinetic response, which is an involuntary eye movement that occurs in response to moving visual stimuli. The system includes a software program and specialized hardware for presenting visual stimuli and recording the animal's responses.
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Lab products found in correlation
3 protocols using optomotry software and apparatus
1
Optokinetic Reflex Tracking Protocol
2
Visual Function Assessment via OKR
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Visual function was assessed by OKR using OptoMotry software and apparatus (Cerebral Mechanics Inc., Medicine Hat, AB, Canada), as in prior studies28 (link). OKR function is determined by the highest spatial frequency at which mice track a 100% contrast grating projected at varying spatial frequencies, and data are reported as cycles/degree.
3
Evaluating Visual Function via Optokinetic Response
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In order to assess the visual function, OptoMotry software and apparatus (Cerebral Mechanics Inc., Medicine Hat, Alberta, Canada) were used to measure the OKR as previously described [14] (link). Briefly, mice were put at the centre of a platform without movement restriction in a closed chamber comprised of four screens and a camera used to detect if they were tracking a 100% contrast grating with varying spatial frequency. The grating started at a spatial frequency of 0.042 cycles/degree and increased progressively. The highest spatial frequency at which mice were able to track correlated with their visual acuity. All data are given in cycles/degree.
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