For determining the antioxidant activities, a 5 g sample of pericarp was homogenized with 25 mL of 95% ethanol, the mixture was centrifuged at 12,000× g and 4 °C for 15 min, and the supernatant was collected and processed for antioxidant measurement. For DPPH radical scavenging activity [35 (link)] of longkong fruit, a 100 µL sample of the extract was thoroughly mixed with 3.9 mL of 60 µmol/L DPPH solution in a test tube. This mixture was then left to incubate for 30 min in darkness at room temperature. The absorbance was measured at 515 nm using a spectrophotometer (model: Mini UV 1240, Shimadzu, Kyoto, Japan). The results were expressed as percentages. For ABTS radical scavenging activity [36 (link)], a 100 µL sample of extract was mixed with 100 µL of ABTS reagent, and then this mixture was incubated for 6 min at ambient temperature. After that, the absorbance readings were taken at 734 nm using a spectrophotometer (model: Mini UV 1240, Shimadzu, Kyoto, Japan). The results were expressed as percentages.
Mini uv 1240
Manufactured by Shimadzu
Sourced in Japan
The Mini UV 1240 is a compact, single-beam UV-Vis spectrophotometer designed for basic absorbance measurements. It features a wavelength range of 190 to 1100 nm and a spectral bandwidth of 2 nm. The instrument provides a simple and easy-to-use interface for routine analysis in the laboratory.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using mini uv 1240
1
Antioxidant Activity Determination of Longkong Fruit
2
Optimization of Cheese Enzymes Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SP Sepharose™ Big Beads (Lot No. 10081054) was purchased from GE Healthcare Bio-Sciences AB, Sweden. Microbial rennet was purchased from Prodinvest Group, Russia. Deoxycholate hydrogen sulfide lactose agar (DHL) (Lot No. 395-00461) was obtained from Shinnihonseiyaku Co., Ltd., Japan. ABTS was purchased from Wako Pure Chemical Industry, Japan. Bovine milk was freshly obtained from the experimental farm at the Faculty of Animal and Agricultural Science, Diponegoro University, Semarang, Indonesia. Culture stock of Escherichia coli FNCC 0009 was purchased from the Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta, Indonesia. A spectrophotometer (Mini UV-1240, Shimadzu, Japan) was used for the Bradford protein analysis and enzyme activity. Sterile syringe filters (Lot No. SF2030813) were purchased from Axiva Sichem Biotech Delhi, India. All chemicals used in this study were of analytical grade.
3
Colorimetric Determination of TPC in Longkong Fruit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The determination of TPC in longkong fruit pericarp was performed using a colorimetric method [34 (link)]. Five grams of the pericarp was homogenized with 25 mL of 95% ethanol, the mixture was centrifuged at 12,000× g and 4 °C for 15 min, and the supernatant was collected and proceeded for the TPC measurement. The procedure involved mixing 8.4 mL of distilled water with 100 μL of the fruit sample and 500 μL of Folin–Cioclateu reagent in a test tube. This mixture was left to stand for 3 min, after which 1.0 mL of 20% sodium carbonate was added. The mixture was then thoroughly vortexed (Vision Scientific Co., Ltd., Daegu, Republic of Korea). After an hour, the absorbance at 720 nm was measured using a spectrophotometer (model: Mini UV 1240, Shimadzu, Kyoto, Japan). The phenolic content was quantified by comparing the absorbance to a standard curve created with chlorogenic acid, and the results were expressed as µg gallic equivalent (GAE) g−1 FW.
4
Quantifying Antioxidant Capacity in Longkong Fruit Pericarp
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The OH− content in longkong fruit pericarp was assessed using the 2-deoxyribose oxidation method by Chung et al. [32 (link)]. A 10 g pericarp sample was homogenized in 50 mL of methanol, followed by filtration and centrifugation at 2000× g for 10 min at 4 °C. The supernatant obtained was then used for analysis. In a test tube, 0.2 mL of a reagent mixture containing 10 mmol of iron (II) sulfate heptahydrate and 10 mmol of ethylenediaminetetraacetic acid (EDTA) was combined with 0.2 mL of 2-deoxyribose. After thorough mixing, 0.8 mL of the sample was added to this mixture, along with 1 mL of 0.1 mol sodium phosphate buffer at pH 7.4, followed by the addition of 200 µL of 10 mmol hydrogen peroxide to the mixture, which was then incubated at 37 °C for 4 h. Subsequently, 1 mL of 2.8% trichloroacetic acid (TCA) solution was added, and the mixture was heated in a boiling water bath for 10 min before cooling to room temperature. The absorbance of the mixture was measured at 520 nm using a UV-Vis spectrophotometer (model: Mini UV 1240, Shimadzu, Kyoto, Japan). The results were then expressed as nmol g−1 FW.
5
Determination of H2O2 in Longkong Fruit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H2O2 content in longkong fruit pericarp was assessed by using the method of Patterson et al. [31 (link)]. A 2 g pericarp sample was homogenized in 15 mL of acetone under cold conditions and then the mixture was centrifuged at 6000× g for 15 min at 4 °C. After that, 1 mL of the supernatant was added along with 0.1 mL of 5% TiOSO4 and 0.2 mL of NH3 to the centrifuge tube and then centrifuged again under similar conditions for 10 min. The resultant pellet was then dissolved in 3 mL of 10% H2SO4 and centrifuged at 5000× g for 10 min at 4 °C. Then, the final supernatant was used to measure the H2O2 content. The measurement was based on the absorbance at 410 nm, using a UV-Vis spectrophotometer (model: Mini UV 1240, Shimadzu, Kyoto, Japan). The results were then expressed as nmol g−1 FW.
6
Solubility Determination of CA Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solubility tests were performed by adding excess amounts of each sample into 10 mL distilled water and stirring it continuously for 48 h at 25 ± 0.5°C. Then, solution samples were centrifuged, filtered, and analyzed for CA content at 242 nm (Shimadzu mini UV 1240, Japan). This test was carried out in triplicate for each formulation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!