Cxcl8

Cytokine concentrations in cell-free supernatants and cell lysates were measured by commercial enzyme-linked immunosorbent (ELISA) kits, specific for: IL-17A (DY317 from R&D systems and 88-7176 from eBioscience), IL-17A/F (88-7117, eBioscience), IL-17B [ABKA2223 from Abnova (Taipei, Taiwan) and ab171344 from Abcam (Cambridge, United Kingdom)], IL-17F (887478, eBioscience), and CXCL8 (Mabtech, Nacka Strand, Sweden). ELISA detection limits were 4 pg/ml (eBioscience) and 15.6 pg/ml (R&D) for IL-17A, 30 pg/ml for IL-17A/F, 24 pg/ml (Abnova) and 10 pg/ml (Abcam) for IL-17B, 16 pg/ml for IL-17F, and 8 pg/ml for CXCL8.

Cytokine concentrations in cell-free supernatants and cell-lysates were measured by commercially available ELISA kits, specific for human: CXCL8 (Mabtech, Nacka Strand, Sweden), CCL23 (R&D Systems, Minneapolis, MN, USA), CCL2 (R&D Systems), CCL3 (eBioscience, San Diego, CA, USA), and CCL4 (eBioscience). Detection limits of these ELISA were: 7.8 pg/ml for CCL23, 7.8 pg/ml for CCL2, 16 pg/ml for CCL3, 4 pg/ml for CCL4, and 16 pg/ml for CXCL8.

Cytokine concentrations in cell-free supernatants and cell-lysates were measured by commercially available ELISA kits, specific for human IL-6 (eBioscience, San Diego, CA, USA), TNFα (eBioscience), G-CSF (R&D Systems), IL-1ra (R&D Systems), CXCL8 (Mabtech, Nacka Strand, Sweden) and IFNα (Mabtech). Detection limits of these ELISA were: 8 pg/ml for IL-6 and IFNα, 4 pg/ml for TNFα, 40 pg/ml for IL-1ra, 16 pg/ml for G-CSF and CXCL8.