Cells and clinical samples were lysed in RIPA buffer, and quantified by Rapid Gold BCA (Thermo, USA). Protein samples were separated equally by SDS-PAGE and electrotransferred to polyvinylidene difluoride (PVDF) membranes, which were then blocked for 1 h, and incubated with anti-DJ-1 (ab76008, Abcam, USA), anti-Bax (ab3191, Abcam, USA), anti-Bcl2 (ab196495, Abcam, USA), Anti-Cleaved Caspase-3(ab214430, Abcam, USA), Anti-CDK4(ab95255, Abcam, USA), Anti-Cyclin D1 (ab226977, Abcam, USA), Anti-E2F1 (ab137415, Abcam, USA), Anti-Cyclin D2 (ab230883, Abcam, USA), Anti-Cyclin D3 (ab112034, Abcam, USA), Anti-RB(17218-1-AP, proteintech, USA), Anti-pRb (phospho S780, ab47763,Abcam, USA), Anti-E2F1(12171-1-AP, proteintech, USA), Anti-H2A(16441-1-AP, proteintech, USA), Anti-α-Tubulin(11224-1-AP, proteintech, USA), Anti-Flag(80010-1-RR, proteintech, USA), and Anti-α-Actin(23660-1-AP, proteintech, USA). Next, the membranes were incubated in corresponding secondary antibodies for 1 h. Proteins were visualized and detected by SuperSignal West Atto(A38554, Thermo, USA).
Ab112034
Manufactured by Abcam
Sourced in United States, United Kingdom
Ab112034 is a laboratory antibody product manufactured by Abcam. It is designed for use in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab226977, by Abcam (1 mentions)
Ab214430, by Abcam (1 mentions)
Ab196495, by Abcam (1 mentions)
Ab3191, by Abcam (1 mentions)
Ab230883, by Abcam (1 mentions)
Ab47763, by Abcam (1 mentions)
Ab137415, by Abcam (1 mentions)
Ab76008, by Abcam (1 mentions)
Rapid gold bca, by Thermo Fisher Scientific (1 mentions)
Anti α actin, by Proteintech (1 mentions)
A38554, by Thermo Fisher Scientific (1 mentions)
Supersignal west atto, by Thermo Fisher Scientific (1 mentions)
Ab236422, by Abcam (1 mentions)
Ab245309, by Abcam (1 mentions)
Ecl detection system, by Cell Signaling Technology (1 mentions)
Ag019, by Beyotime (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
2 protocols using ab112034
1
Protein Expression Analysis of DJ-1, Apoptosis, and Cell Cycle Regulators
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein was extracted from tissues and cells for analysis. The samples were boiled at 100 °C for 5 min. Equal amounts of protein were separated by SDS-PAGE followed by transfer to PVDF membranes (Millipore, USA). The membranes were incubated with primary antibodies overnight at 4 °C. The following primary antibodies: mouse anti-human TRIM44 (ab236422; 1:1,000 dilution; Abcam, Cambridge, UK), rabbit anti-human CCND3 (ab112034, 1:1,000 dilution; Abcam, Cambridge, UK), rabbit anti‐human FOXM1 (ab245309, 1:1,000 dilution, Abcam, Cambridge, UK), rabbit anti-human EZH2 (#4905,1:100 dilution, Cell Signaling Technology), rabbit anti-human CCNE2 (ab226388, 1:1,000 dilution; Abcam, Cambridge, UK), rabbit anti-human BIRC5 (PAB18224, 1:1,000 dilution; Abnova, China), and mouse anti-GADPH (AG019, 1:1,000 dilution, Beyotime) as internal controls. Immunoreactive protein bands were detected using an ECL detection system (Cell Signaling Technology, USA).
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