Log In Sign up
The largest database of trusted experimental protocols

Actin hrp antibody

Manufactured by Santa Cruz Biotechnology
Visit Supplier

The Actin- HRP antibody is a horseradish peroxidase-conjugated antibody that specifically binds to actin, a cytoskeletal protein found in eukaryotic cells. This antibody can be used as a detection tool in various immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Enhanced chemiluminescence western blot substrate, by Thermo Fisher Scientific (1 mentions) Aurka, by Cell Signaling Technology (1 mentions) Hrp conjugated anti mouse or anti rabbit secondary antibody, by Bio-Rad (1 mentions) Las4000 imager, by GE Healthcare (1 mentions) Westernbright ecl, by Advansta (1 mentions) Fibrinogen β fgb, by Santa Cruz Biotechnology (1 mentions) Tsg101, by Santa Cruz Biotechnology (1 mentions)

4 protocols using actin hrp antibody

1

Western Blot Analysis of FIRRE Overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Control empty vector or FIRRE overexpressed cells were lysed in a sample buffer, subjected to SDS-PAGE, and transferred onto a nitrocellulose membrane. The membrane was blocked in 5% nonfat dried milk and incubated with a specific primary antibody overnight at 4 °C followed by incubation with a secondary antibody conjugated with horseradish peroxidase (HRP) for 1 h. Proteins were detected by an enhanced chemiluminescence Western blot substrate (ThermoFisher Scientific). Commercially available antibodies to cyclin D1 (Santa Cruz Biotechnology Cat# sc-8396, 1:1000) and HuR (Santa Cruz Biotechnology Cat# sc-5261, 1:1000), TTP (Sigma-Aldrich Cat# T5327, 1:1000), Actin- HRP antibody (Santa Cruz Biotechnology Cat# 517582, 1:5000), and tubulin (Santa Cruz Biotechnology Cat# sc-58666, 1:2000) were used for Western blot analysis.
Haga Y., Bandyopadhyay D., Khatun M., Tran E., Steele R., Banerjee S., Ray R., Nazzal M, & Ray R.B. (2024). Increased expression of long non-coding RNA FIRRE promotes hepatocellular carcinoma by HuR-CyclinD1 axis signaling. The Journal of Biological Chemistry, 300(5), 107247.
+ Open protocol
+ Expand
2

Western Blot Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were prepared using 2× SDS sample buffer, and Western blot analysis was performed using specific antibodies to AURKA (1: 1000, Cell Signaling Technology, CST), p-CDK1/p-Cdc2 (Tyr-15) (1: 1000, CST), total CDK1/Cdc2 (1: 1000, CST), CTCF (1: 1000, CST), and FOXM1 (1: 1000, CST). The HRP-conjugated anti mouse or anti-rabbit secondary antibodies (1:5000) were purchased from Bio-Rad. The blot was reprobed with Actin- HRP antibody (1:5000, Santa Cruz Biotechnology, SBT) to compare protein load in each lane. Densitometry analysis was performed using Image J software.
Sur S., Steele R., Ko B.C., Zhang J, & Ray R.B. (2022). Long noncoding RNA ELDR promotes cell cycle progression in normal oral keratinocytes through induction of a CTCF-FOXM1-AURKA signaling axis. The Journal of Biological Chemistry, 298(5), 101895.
+ Open protocol
+ Expand
3

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gel electrophoresis was in Nu-Page 4-12% gradient Bis-Tris NuPage gels (Life Technologies) followed by transfer to nitrocellulose. Mouse pan-specific antibody 9A7 (gift of Dr. Maureen McEnery, Case Western Reserve University, Cleveland, OH) was employed to detect the total amount of alpha subunit isoforms. Monoclonal antibodies 6F (Developmental Studies Hybridoma Bank) or M17-P5-F11 (gift of Dr. W. James Ball, University of Cincinnati) were used to detect human α1 and β2 subunits of Na,K-ATPase, respectively. α3 subunit of Na,K-ATPase was detected with the goat peptide-directed antibody C16 (Santa Cruz Biotechnology). Actin-HRP antibody was from Santa Cruz Biotechnology. Secondary antibodies were HRP-conjugated, and final detection was with chemiluminescence (WesternBright ECL, Advansta, CA). An LAS 4000 imager (GE Healthcare Life Sciences) with ImageQuant software was used for the analysis of Western blots. All analyses were performed with a minimum of 3 biological replicates, and actin was used as a loading control for all samples for quantification. When protein induction is large compared to the basal level, the accuracy of blot quantification is limited by the accuracy of background subtraction: three different ImageQuant automatic background subtraction methods were employed, and the results averaged for a best-estimate of expression differences.
Arystarkhova E., Haq I.U., Luebbert T., Mochel F., Saunders-Pullman R., Bressman S.B., Feschenko P., Salazar C., Cook J.F., Demarest S., Brashear A., Ozelius L.J, & Sweadner K.J. (2019). Factors in the disease severity of ATP1A3 mutations: impairment, misfolding, and allele competition. Neurobiology of disease, 132, 104577.
+ Open protocol
+ Expand
4

Extracellular Vesicle Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted February 9, 2021. ; https://doi.org/10.1101/2021.02.08.430369 doi: bioRxiv preprint Cells lysates were subjected to Western blot analysis using specific antibodies to CD63 (Santa Cruz Biotechnology), TSG101 (Santa Cruz Biotechnology), tenascin (TNC) (Sigma), fibrinogenβ (FGB) (Santa Cruz Biotechnology), phospho-NF-kB p65 (Ser536) (Cell Signaling Technology, CST), NF-kB p65 (CST). The blot was reprobed with actin-HRP antibody (Santa Cruz Biotechnology) to compare protein load in each lane. Densitometry analysis was done using Image J software.
Sur S., Khatun M., Steele R., Isbell T.S., Ray R.B., & Ray R.B. (2021). Exosomes from COVID-19 patients carry tenascin-C and fibrinogen-β in triggering inflammatory signals in distant organ cells.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!