Human insulin elisa kit 10 1113 01

At 28 days post‐transplant, all mice underwent an intraperitoneal glucose tolerance test. After 4 h of fasting, two grams of glucose (dissolved in phosphate‐buffered saline) per kilogram body weight was injected into the peritoneum. Blood samples were taken from the tail vein at 0, 15, 30, 60, and 120 min after glucose administration for the measurement of glucose and insulin concentrations. Plasma insulin concentration was determined analogously to the GSIS measurements (Human Insulin ELISA Kit 10‐1113‐01; Mercodia AB, Uppsala, Sweden).

In vitro functionality of isolated islets (in duplo of ±20 islets) was tested after one day of culture via a dynamic glucose‐stimulated insulin secretion (GSIS) test using a Brandel Suprafusion 1000 System (Brandel, Gaithersburg, USA), as previously described [27 (link)]. The insulin concentration at each time point was then divided by the average insulin concentration (Human Insulin ELISA Kit 10‐1113‐01; Mercodia AB, Uppsala, Sweden) of the last three insulin concentrations during the second low‐glucose phase. This stimulation index per time point was averaged for HMP and SCS pancreases. To calculate the area under the curve of the stimulation indices, the stimulation index curves were integrated over time.