Dmem glutamax 4.5 g l glucose

Human Embryonic Kidney (HEK293) cells were grown in Dulbecco’s Modified Eagle medium (DMEM) Glutamax 4.5 g/l glucose (Gibco, Invitrogen) supplemented with 10% (v/v) of Fetal Bovin Serum (Gibco, Invitrogen) and 1% (v/v) of Penicillin-Streptomycin solution (Gibco, Invitrogen) at a temperature of 37 °C in the presence of 5% of CO₂.

HEK293 were grown in Dulbecco’s Modified Eagle medium (DMEM) 1g/l glucose and glutamine (Gibco, Invitrogen) supplemented with 10% (v/v) of Fetal Bovin Serum (FBS) (Gibco, Invitrogen) and 1% (v/v) of Antibiotic-Antimycotic solution (A-A) (Gibco, Invitrogen) at a temperature of 37°C in the presence of 5% of CO₂. For immunofluorescence staining and intracellular accumulation assays, HEK293 cells were grown in DMEM Glutamax 4.5 g/l glucose (Gibco, Invitrogen) supplemented with 10% (v/v) FBS and 1% (v/v) A-A.
K562 were grown in Iscove’s Modified Eagle Dulbecco’s medium (IMDM) (Gibco, Invitrogen) supplemented with 10% (v/v) of FBS (Gibco, Invitrogen) and 1% (v/v) A-A (Gibco, Invitrogen) at a temperature of 37°C in the presence of 5% of CO₂. For intracellular accumulation assay, K562 cells were grown in IMDM Glutamax (Gibco, Invitrogen), 10% (v/v) FBS and 1% (v/v) A-A.