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2 protocols using rabbit anti phospho erk 1 2 thr tyr 202 204

1

Antibody Panel for EGFR Signaling Analysis

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Mouse anti-HA (MMS-101P, Biolegend, San Diego, CA), mouse anti-phospho tyrosine (PY72) (P172.1, InVivo Biotech Services, Henningsdorf, Germany), rabbit anti-c-Cbl (sc-170, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-α-Tubulin (Sigma Aldrich, St. Louis, MO); living colors rabbit anti-GFP (632593, Clontech, Mountain View, CA), living colors mouse anti-GFP (632681, Clontech, Mountain View, CA), mouse anti-Rab11 (610656, BD Biosciences, Heidelberg, Germany), rabbit anti-Rab11a (ab65200, Abcam), rabbit anti-Rab11 (3539, Cell Signaling Technology, Danvers, MA), rabbit EGFR (4267, Cell Signaling Technology, Danvers, MA), rabbit pY1045 (2237, Cell Signaling Technology, Danvers, MA), rabbit pY1068 (3777, Cell Signaling Technology, Danvers, MA), goat EGFR (AF231, R&D Systems, Minneapolis, MN), mouse pY845 (558381, BD Biosciences, Heidelberg, Germany), mouse anti-Rab5 (610281, BD Biosciences, Heidelberg, Germany), rabbit anti-phospho ERK-1/2 Thr/Tyr 202/204 (9101, Cell Signaling Technology, Danvers, MA), mouse anti-ERK1/2 (Ab366991, Abcam); rabbit anti phosphor-Akt Ser473 (9271, Cell Signaling Technology, Danvers, MA), mouse anti-Akt (pan) (2920, Cell Signaling Technology, Danvers, MA).
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2

Antibody Reagents for Cellular Signaling

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rabbit anti‐RPTPγ (rb‐RPTPγ‐P4: gift from C. Sorio, Department of Pathology, University of Verona, Verona, Italy, (Sorio et al, 1995 (link))), mouse anti‐TCPTP (MAB1930, R&D Systems, Minneapolis, MN), mouse anti‐p22phox (ab80896, Abcam, Cambridge, UK), anti‐cysteine sulfenic acid 2‐thiodimedone (ABS30, Merck, Darmstadt, Germany), living colors mouse anti‐GFP (632381, Clontech, Mountain View, CA), goat anti‐GFP (ab5450, Abcam), rabbit anti‐phospho EGFR Y1068 (3777, Cell Signaling Technology, Danvers, MA), mouse anti‐phospho EGFR Y1068 (2236, Cell Signaling Technology) rabbit anti‐EGFR (4267, Cell Signaling Technology), goat anti‐EGFR (AF231, R&D Systems), rabbit anti‐phospho‐ERK‐1/2 Thr/Tyr 202/204 (9101, Cell Signaling Technology), mouse anti‐ERK1/2 (ab366991, Abcam), rabbit anti‐phospho‐Akt Ser473 (9271, Cell Signaling Technology), mouse anti‐Akt (pan) (2920, Cell Signaling Technology), rabbit anti‐EEA1 (3288, Cell Signaling Technology), rabbit anti‐Rab7 (9367, Cell Signaling Technology), rabbit anti‐Rab11a (2413, Cell Signaling Technology), rabbit phospho‐Rb Ser807/811 (8516, Cell Signaling Technology), mouse anti‐GAPDH (CB1001, Merck), mouse anti‐α‐Tubulin (T6074, Merck), mouse anti‐Na+/K+ ATPase‐α3‐subunit (BML‐SA247‐0100, Enzo Life Sciences, NY).
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