Recombinant human tgf α

Manufactured by R&D Systems

Recombinant human TGF-α is a protein that is produced using recombinant DNA technology. It is a member of the epidermal growth factor family and plays a role in cell growth and differentiation.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Soybean trypsin inhibitor, by Thermo Fisher Scientific (1 mentions) Caerulein, by Merck Group (1 mentions) Rat tail collagen 1, by BD (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Santa Cruz Biotechnology (1 mentions) Ectoine, by Merck Group (1 mentions) Erlotinib, by Selleck Chemicals (1 mentions) Azd9291, by Selleck Chemicals (1 mentions) Inh 172, by Merck Group (1 mentions) Tapi 1, by Selleck Chemicals (1 mentions) Vx 661, by Selleck Chemicals (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Forskolin, by Abcam (1 mentions)

3 protocols using recombinant human tgf α

Caerulein-Induced Pancreatic Injury Model

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Caerulein, DMSO and all other chemicals unless specified were obtained from Sigma (St. Louis, MO). The GSK-3 inhibitor 9-ING-41 (GSK-3i) was obtained from Actuate Therapeutics (Fort Worth, TX). Recombinant human TGF-α was obtained from R&D Systems (Minneapolis, MN). Soybean trypsin inhibitor was obtained from Affymetrix (Santa Clara, CA). Rat-tail collagen I was obtained from BD (Franklin Lakes, NJ). AR42J cells were passaged in Ham’s F-12K (Kaighn’s) medium supplemented with 15% fetal bovine serum (FBS), 2 mM L-glutamine and penicillin/streptomycin. For Caerulein stimulation experiments, cells were serum starved for 16 hours and pre-treated with 9ING41 (500 nM) for 2 hours, then 10 nM of Caerulein for 2 or 6 hours. Stable shGSK-3β or scramble AR42J cells were fasted overnight prior to Caerulein (10 nM) stimulation. Cells were harvested 2, 6, and 12 hours post Caerulein stimulation.

Ding L., Liou G.Y., Schmitt D.M., Storz P., Zhang J.S, & Billadeau D.D. (2017). Glycogen synthase kinase-3β ablation limits pancreatitis induced acinar-to-ductal metaplasia.. The Journal of pathology, 243(1), 65-77.

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HSP20 Phosphorylation Signaling Pathways

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HSP20 antibodies were purchased from Enzo Life Sciences Inc. (Farmingdale, NY). Antibodies recognizing phosphorylated serine 16 in HSP20 were constructed as described previously [29 ], or obtained from Abcam plc (Cambridge, UK). Antibodies against phospho-JNK and phospho-EGFR (Y1068) were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Recombinant human TGF-α was obtained from R&D systems Inc. (Minneapolis, MN). SP600125 was purchased from Calbiochem-Novabiochem Co. (La Jolla, CA). SP600125 was dissolved in dimethyl sulfoxide. The maximum concentration of dimethyl sulfoxide was 0.1%, which did not affect the cell migration assay or Western blot analysis. N⁶,2’-O-dibutyryladenosine 3,5’-cyclic monophosphate (dibutyryl cAMP) was purchased from Sigma-Aldrich Co. (St Louis, MO). The BCA protein assay kit was obtained from Thermo Fisher Scientific Inc. (Waltham, MA). All other materials and chemicals were obtained from commercial sources.

Matsushima-Nishiwaki R., Toyoda H., Nagasawa T., Yasuda E., Chiba N., Okuda S., Maeda A., Kaneoka Y., Kumada T, & Kozawa O. (2016). Phosphorylated Heat Shock Protein 20 (HSPB6) Regulates Transforming Growth Factor-α-Induced Migration and Invasion of Hepatocellular Carcinoma Cells. PLoS ONE, 11(4), e0151907.

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CFTR Inhibitor and Kinase Inhibitor Assay

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The CFTR inhibitor Inh-172 was purchased from Sigma-Aldrich (St. Louis, MO). UO126, was from Calbiochem (La Jolla, CA). Epidermal growth factor receptor (EGFR) inhibitors, AZD9291 and erlotinib, TAPI-1, and CFTR corrector VX-661 were purchased from Selleckchem (Houston, TX). Forskolin was purchased from Abcam (Cambridge, MA). Recombinant human TGF-α was purchased from R&D Systems (Minneapolis, MN). Ectoine and cycloheximide were purchased from Sigma-Aldrich (St. Louis, MO).

Wellmerling J., Rayner R., Chang S.W., Kairis E.L., Kim S.H., Sharma A., Boyaka P.N, & Cormet-Boyaka E. (2022). Targeting the EGFR-ERK axis using the compatible solute ectoine to stabilize CFTR mutant F508del. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36(5), e22270.

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