Fitc conjugated mouse anti human cd34

hPDLSCs were stained with one of the following monoclonal antibodies (all from eBiosciences, San Diego, CA, USA): phycoerythrin (PE)-conjugated mouse anti-human CD29, PE-conjugated mouse anti-human CD90, PE-conjugated mouse anti-human CD105, PE-conjugated mouse anti-human CD146, PE-conjugated mouse IgG1 K isotype control, fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD14, FITC-conjugated mouse anti-human CD31, FITC-conjugated mouse anti-human CD34, FITC-conjugated mouse anti-human CD45, FITC-conjugated mouse IgG1 K isotype control.

FITC-conjugated mouse anti-human CD34 and mouse IgG1 K isotype control-FITC were from eBioscience (CA, USA). APC-conjugated mouse anti-human VEGFR-3 and mouse IgG1 isotype control-APC were from R&D systems (USA). Anti-LYVE-1 antibody was from Abcam. FITC-conjugated secondary antibodies were from KeyGEN Biotechnology Company (Nanjing, China).
Ficoll-Hypaque solution was purchased from Haoyang Biotechnology Company (Tianjin, China). Goat serum and 4% paraformaldehyde were from Boster Biotechnology Company (Wuhan, China). VEGF-C and SDF-1 were from PeproTech. Lenvatinib (E7080) was from Selleckchem. AMD3100 was from Sigma-Aldrich. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were from Hyclone. Complete growth medium EGM-2MV was from Lonza. 4′,6-Diamidino-2-phenylindole (DAPI) was from Beyotime Biotechnology Company (Shanghai, China). JFK (Lot number 130202) was obtained from Jilin Jinfukang Pharmaceutical Co., Ltd., (Jilin, China).

Phycoerythrin (PE)-conjugated mouse anti-human CD73, fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD90, PE-conjugated mouse anti-human CD105, and FITC-conjugated mouse anti-human CD34 antibodies were purchased from eBioscience (Waltham, MA, USA). MACS Plex Exosome Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) was used to evaluate the exosome surface markers (CD63 and CD81). PE-conjugated mouse anti-human IDO was treated after permeabilization with 0.1% Triton X-100 in PBS for 10 min. All data were measured with the Acoustic Focusing Cytometer (Thermo Fisher Scientific, Waltham, MA, USA).